Ibuprofen and indomethacin are clinically important inhibitors of prostaglandin H₂ synthase-1. Cells expressing this enzymewere incubated under four different conditions, after which the activity of the enzyme was measured by adding radiolabeledarachidonic acid and detecting newly-produced prostaglandin H₂:1. 40 min without inhibitor (control)2. 40 min with inhibitor3. 40 min with inhibitor, after which the cells were resuspended in medium without inhibitor4. 40 min with inhibitor, after which the cells were resuspended in medium without inhibitor and incubated for an additional30 minThe bar graphs show the results of the experiments with ibuprofen and indomethacin. The bars in each graph correspond to thefour different incubation conditions.IbuprofenProstaglandin H₂ synthesized(relative to control)10080604020III(1) (2)ll(3)(4)Prostaglandin H₂ synthesized(relative to control)100k80604020I(1)Indomethacin(2)(3)(4) Which hypothesis best explains the results for these two inhibitors?Ibuprofen and indomethacin both covalently modify prostaglandin H₂ synthase-1.Ibuprofen dissociates slowly from prostaglandin H₂ synthase-1, whereas indomethacin dissociates rapidly.Ibuprofen dissociates rapidly from prostaglandin H₂ synthase-1, whereas indomethacin dissociates slowly.Ibuprofen and indomethacin both dissociate rapidly from prostaglandin H₂ synthase-1.How would these results look if aspirin were tested in a similar fashion? Complete the bar graph by dragging a bar to eachcolumn. Some bars may be used more than once and some may not be used at all.Prostaglandin H₂ synthesized(relative to control)10080604020I.(1)Aspirin(2)(3)(4)Answer Bank

Inhibitors can be broadly categorized into reversible and irreversible.Reversible inhibitors bind…

Answered: Which hypothesis best explains the…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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The following kinetic data were collected for prostaglandin endoperoxide synthase, an enzyme involved in pain and inflammation. Using data in the first two columns, use a graphical analysis to determine the Vmax and the Km of the enzyme. Ibuprofen (Advil) is an inhibitor of prostaglandin endoperoxide synthase. By inhibiting this enzyme, ibuprofen helps to reduce pain and fever. Using the data in the first and third columns, determine the type of inhibition that ibuprofen exerts on prostaglandin endoperoxide synthase. In the presence of the inhibitor, determine the Vmax and the Km of the enzyme. [S] mM Product formed Product formed (mmol/min) with 10 mg/mL ibuprofen mmol/min) 0.5 23.5 16.67 1.0 32.2 25.25 1.5 36.9 30.49 2.5 41.8 37.04 3.5 44.0 38.91
An experiment was carried out to measure the reaction rate of hydrolysis of acetylcholme (substrate) with serum enzymes (Eadie, 1949). In the experiment, two experiments were conducted, namely experiment 1 without using a prostigmine inhibitor and experiment 2 using a prostigmine inhibitor at 1.5 x 10^-7 mol/l. the data obtained are: a. Is prostigmine competitive or noncompetitive inhibitor? b. determine the value of km and rmax for the two experiments, compare
One of your colleagues has obtained a sample of muscle phosphorylase b that is known to be relatively inactive. She has approached you for advice on how to set up an appropriate assay. She has the following items available, not all of which are appropriate for this study. Help her out by selecting the items that she should use and what their purpose is in the assay, and then explain why each of the other items would not be useful. 1. 100 UM AMP 2. 100 UM GTP 3.100 uM glucose 4. 100 uM glucose 6-phosphate 5. Branched glycogen 6. Amylose (i.e. unbranched glycogen) 7.50 mM HEPES buffer, pH 7.5 8. 50 mM potassium phosphate buffer, pH 7.5 Write out a short explanation for each of the above items, and upload your answers by the due date.
A series of novel phenadoxone derivatives without mu2 receptor activity (mu2 activity is responsible for physical dependence) proposed to be developed as analgesics is shown below. Addition of which heterocyclic substituent R to phenadoxone is LIKELY to cause the MOST binding of the corresponding derivative to plasma proteins? Use the additivity of approximate estimates of logP to answer this question. phenadoxone derivatives A. Azetidine B. Thiophene C. Oxetane D. Furan E. Pyrrole ترف لي تي في R= -NH
The Warburg effect is where tumor cells preferentially catabolize glucose to lactate even in the presence of oxygen (aerobic glycolysis), whereas normal cells preferentially catabolize glucose to carbon dioxide (oxidative phosphorylation). Treatment of tumor cells with the lactate dehydrogenase inhibitor NCI-737 blocks ATP production. Which of the following statements about NCI-737 treated tumor cells are MOST correct? 1) AGglycolysis is negative 2) AGglycolysis is positive 3) AG" for glycolysis is negative 4) AG" for glycolysis is positive 5) NAD+/NADH decreases 6) NAD+/NADH increases 1, 4, 6 1, 3, 5 2, 4, 6 2, 3, 6 2, 3, 5
Which arrestin is required for activation of phosphorylation of MDM2 during chronic Iso stimulation? use the following Figures 1and 2 respectively to explain please
Inhibitors of acetylcholinesterase, such as edrophonium, are used to treat Alzheimer’s disease. The substrate for acetylcholinesterase is acetylcholine. Structures are attached. What kind of inhibitor is edrophonium? Explain. Can inhibition by edrophonium be overcome in vitro by increasing the substrate concentration? Explain. Does this inhibitor bind reversibly or irreversibly to the enzyme? Explain.
Staphylococcal nuclease has a ΔΔG‡ of -84.1 kJ mol-1 at 25.0 °C. If the uncatalyzed rate is 0.630x10-13 µmol s-1, calculate the enzyme-catalyzed rate in µmol s-1. (Use R = 8.3145 J mol-1 K-1)
Calculate the slope on a Lineweaver-Burk plot (Km / Vmax) for the lactase reaction with inhibitor X. (inhibitor X changes lactase activity to a Vo of 0.10 mM per minute when [S] = 1.0 mM, and a Vo of 0.133333333333 mM per minute when [S] = 2.0 mM) 0.20 per minute 0.50 per minute 1.0 per minute 2.0 per minute 5.0 per minute
answer the following questioms.. 1.What is the action of invertase?2.What is the reagent used to detect glucose in the detection of invertase? What is its principle?
Tyrosinase enzyme activity is assayed by monitoring the oxidation of 3, 4-dihydroxyphenylalanine (dopa) to the red-colored dopachrome. Calculate the tyrosinase activity (U/mL) by using the experimental data given below
Under what conditions, a higher rate in the presence of the inhibitor observed? (how could the effect of inhibitor be compensated) explain

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