Biochemistry: The Molecular Basis of Life
6th Edition
ISBN: 9780190209896
Author: Trudy McKee, James R. McKee
Publisher: Oxford University Press
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Chapter 1, Problem 17RQ
Summary Introduction
To review:
Definition of the following biological terminologies:
1. System
2. Network
3. Emergent property
4. Degeneracy
5. Feedback control
Introduction:
A cell is a biological degenerate system, in which a number of events occur in the form of a continuous network. It consists of several structural and functional entities, which operate in a systematic way to carryout different functions necessary for the survival of the cell.
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Question 1.
Enzymes, proteins and deoxyribonucleic acid (DNA) are important biological
macromolecules. Enzymes are not only speed up the reaction, but also are necessary for DNA
reproduction.
c) Describe an example of biomedical application that utilizes a protein folding technique.
d) Describe the effect of pH on the oxygen binding affinity of hemoglobin.
Question 1.
Enzymes, proteins and deoxyribonucleic acid (DNA) are important biological
macromolecules. Enzymes are not only speed up the reaction, but also are necessary for DNA
reproduction.
e) Michaelis - Menten Kinetics model explains how reaction rates are depending on the
concentration of enzyme and substrate.
i.
What is meant by the saturation of enzyme?
ii. A decrement in enzyme activity is known as enzyme inhibition. Based on normal
enzyme activity, explain the enzyme kinetics for competitive inhibition and
noncompetitive inhibition, as shown in Figure 1.
Normal
enzyme
Competitive inhibitor
Noncompetitive inhibitor
Substrate concentration
Figure 1: Enzyme inhibition
f) What is the importance of cofactor in the enzyme activity? Give an example of a disease
that is related with cofactors.
Rate of reaction
Question 4.
Diabetes mellitus possesses a serious health challenge worldwide and there has been an
increased awareness for self-monitoring and point of care testing. The development of self-
monitoring of blood glucose (SMBG) is probably the most important advance in controlling
diabetes since the discovery of insulin.
c) Compare the sensitivity and accuracy of glucose meter with a spectrophotometer.
d) Elaborate the limitation of glucose meter in diagnostic application?
e) Examine the advantage of DNA based biosensor as compared to enzyme biosensor
Chapter 1 Solutions
Biochemistry: The Molecular Basis of Life
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