Page 17 Use the Michaelis-Menten equation to calculate the missing values of [S] given below if Vmax = 5 mmol/min. Y [S] K + [S] P: If necessary, you may plot [S] versus v. (not the reciprocals!). Draw a line parallel to the X-axis near Vmax (asymptote) and extend your plotted line to show its approach to Vmax- [S] (mm) 10 [S] ₁ [S]2 [S]3 [S]4 Vo (mmol/min) 1.2 1.7 2.1 2.2 2.5
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- The graph of the Michaelis-menten equation, vo against [So] is unsatisfactory as a means of determining Vmax and Km. Briefly discuss why this is the case. How did lineweaver and buck overcome this problem?Using the formula V₁D₁ = V₂D2, what volume of a 1/100 (10-2) dilution would you need to produce 100 mL of a 1/10000 (10-4) dilution? (Note: provide your answer in decimal format to three decimal places.)20.28 The following initial velocities were determined spec- trophotometrically for solutions of sodium succinate to which a constant amount of succinoxidase was added. The velocities are given as the change in absorbancy at 250 nm in 10 s. Calculate V, KM, and K, for malonate. AX 103 10 s 15 x 10-6 mol L-1 [Succinate] 10-3 mol L-1 No Inhibitor Malonate 10.0 16.7 14.9 2.0 14.2 10.0 1.0 11.3 7.7 0.5 8.8 4.9 0.33 7.1 Hint: Treat this problem using the competitive inhibited rate law
- A Br selective electrode is applied for measuring bromide ions in saline solution. Cl' ions were found to interfere, with a kPot = 0.0050. %3D After calibration, the ISE equation was determined to be y = -70 mV - 59.2mV*log(x). If we measure a potential of 93 mV, and the [CI]=0.100 M, what was the [Br'] in units of M? O 0.00821 O 0.100 O 0.0050 O 0.123 O Correct answer not found in other optionsCan you please calculate the following?Explain the limitations of the following expressions: (a) DS = C ln(Tf /Ti), (b) DG = DH − TDS, and (c) DG = wmax,non-exp.
- 6 x 10⁻⁵¹ = (0.15 M)² [S²⁻] [S²⁻] = (6 x 10⁻⁵¹) / (0.15 M)² Calculating, we find the minimum concentration of sulfide ion required: [S²⁻] ≈ 2.7 x 10⁻⁴⁹ M. check it calcution is writeCardiac output of animals can be characterized in L∙kg-1∙min-1. Explain the need in adding the kg units to this formula.A 0.0284 M aqueous solution of lactic acid, HC3H5O3, a substance that accumulates in the blood and muscles during physical activity, is found to be 6.7% ionized. Determine the value of Ka for lactic acid.
- Consider a suspension of particles (isoelectric point is at pH 6) in water at pH 2 and a NaCl concentration of 0.001 M. Describe how the strength of repulsion varies with the following changes, assuming all other conditions remain constant. Give a description (more than just increase or decrease) in terms of the effect on the double layer thickness and the zeta potential. (a) Change from 0.001 M NaCl to 0.1 M NaCl, (b) Change from pH = 2 to pH = 5.For the following Rx: +4.50 -1.25 X 070, what is the sphere power at 160? O +1.25D O-3.25D +3.25D O-1.25DA research group discovers a new version of happyase, which they call happyase*, that catalyzes the chemical reaction The researchers begin to characterize the enzyme. (a) In the first experiment, with [Et] at 4 nM, they find that the Vmax is 1.6 uM/s. Based on this experiment, what is the kcat for happyase*? (Include appropriate units.) (b) In another experiment, with [Et] at 1 nM and [HAPPY] at 30 uM, the researchers find that V0 = 300 nM/s. What is the measured Km of happyase* for its substrate HAPPY? (Include appropriate units.) (c) Further research shows that the purified happyase* used in the first two experiments was actually contaminated with a reversible inhibitor called ANGER. When ANGER is carefully removed from the happyase* preparation and the two experiments repeated, the measured Vmax in (a) is increased to 4.8 uM/s, and the measured Km in (b) is now 15 uM. Based on this information, can you figure out what type of inhibitor is ANGER? (Use table 6.9 at the end of the…