Biochemistry: Concepts and Connections (2nd Edition)
2nd Edition
ISBN: 9780134641621
Author: Dean R. Appling, Spencer J. Anthony-Cahill, Christopher K. Mathews
Publisher: PEARSON
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Textbook Question
Chapter 2, Problem 21P
Is RNA-binding enzyme RNase A more likely to have a pl of 9.2 or 5.0? Briefly explain your reasoning.
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Normal hemoglobin (HbA)
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Protein 2-10092015113649.pdf
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Name
Class
Date
Interpreting Diagrams: Understanding the Main Ideas
The Genetic Code (MRNA)
Lysine
Lysine
Asparagine
Asparagine
Arginine
Arginine
Serine
Serine
Isoleucine
Methionine
Isoleucine
Isoleucine
Threonine
Threonine
Threonine U
Threonine c
Glutamic acid Glycine
Glutamic acid Glycine
Aspartic acid Giycine
Aspartic acid Glycine
Valine
Valine
Valine
Valine
Alanine
Alanine
Alanine
Alanine
"Stop" codon "Stop" codon Leucine
Trytophan
Cysteine
Cysteine
Al
Gl
"Stop" codon
Tyrosine
Тугosine
Serine
Serine
Phenylalanine Serine
Phenylalanine Serine
Leucine
Glutamine
Giutamine
CHistidine
Histidine
Arginine
Arginine
Arginine
Arginine
Al
Leucine
Leucine
Leucine
Loucine
Proline
Proline
Proline
Proline
Icl
A
G
Second Base in Code Word
Use the information in the accompanying figure to complete the following
table. The first row has been completed to help you get started.
DNA codon
MRNA codon
IRNA Anticodon
Amino…
Which of the three 20-amino-acid sequences listed below in the single-letter amino acid code is the most likely candidate to form a transmembrane region (α helix) of a transmembrane protein? Explain your answer.A. I T L I Y F G N M S S V T Q T I L L I SB. L L L I F F G V M A L V I V V I L L I AC. L L K K F F R D M A A V H E T I L E E S
Chapter 2 Solutions
Biochemistry: Concepts and Connections (2nd Edition)
Ch. 2 - Suppose a chloride ion and a sodium ion are...Ch. 2 - Draw two different possible hydrogen-bonding...Ch. 2 - Prob. 3PCh. 2 - 4. What is the pH of each of the following...Ch. 2 - Prob. 5PCh. 2 - The weak acid HA is 2% ionized (dissociated) in a...Ch. 2 - 7. Calculate the pH values and draw the titration...Ch. 2 - What is the pH of the following buffer mixtures?...Ch. 2 - a. Suppose you wanted to make a buffer of exactly...Ch. 2 - Prob. 10P
Ch. 2 - You need to make a buffer whose pH is 7.0, and you...Ch. 2 - Describe the preparation of 2.00 L of 100 glycine...Ch. 2 - Carbon dioxide is dissolved in blood (pH 7.4) to...Ch. 2 - What is the molecular basis for the observation...Ch. 2 - The anno acid arginine ionizes according to the...Ch. 2 - It is possible to make a buffer that functions...Ch. 2 - A student is carrying out a biological preparation...Ch. 2 - Histidine is an amino acid with three titratable...Ch. 2 - Prob. 19PCh. 2 - A biochemical reaction takes place in a 1.00 ml...Ch. 2 - Is RNA-binding enzyme RNase A more likely to have...Ch. 2 - Consider a protein in which a negatively charged...Ch. 2 - Prob. 23PCh. 2 - Prob. 24P
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- 4.08 2.54 H H 1.02 1.02 1.67 E E = EXON = INTRON E 10.8 kbp 3.94 3.66 E = EcoRI site wwwwwwwww H = Hindill site HI Н 10.31t E E 1.76 1.10 Note: Fragment sizes are not to scale and all fragment lengths are in kilobase pairs (kbp) The a. Indicate what enzymes you would use for digestion and the gel electrophoresis pattern of the restriction fragments (draw a diagram of the gel with fragment positions, indicate field polarity, and direction of migration). Please do single and a double digest of the DNA. Grammarlyarrow_forwardIn the folded protein, His108 forms a salt bridge with Asp44. The pKa of the imidazole functional group in the side chain of histidine is 6.0. The pKa of the carboxylic acid functional group in the side chain of aspartate is 4.1. How do you think the pKa of the side chain of His108 will change in the folded protein? a. The pKa will be > 6.0. b. The pKa will be < 6.0. c. The pKa will not change. Use chemical reasoning to justify your answer.arrow_forward1₂ 4.08 H Note: 1.02 2.54 1.02 1.67 E E = EXON H = INTRON Î E 10.8 kbp 3.94 3.66 E = EcoRI site H = Hindill site HI 10.sht E E 1.76 1.10 Fragment sizes are not to scale and all fragment lengths are in kilobase pairs (kbp) Describe how you can determine if the gene is interrupted and, if so, the number of interruptions by restriction endonuclease analysis and Southern analysis. Iarrow_forward
- The bacterially produced antibiotic gramicidin A forms channels in cell membranes that allow the free diff usion of Na+ and K+ ions, thereby killing the cell. This peptide consists of a sequence of Dand L-amino acids. The sequence of a segment of fi ve amino acids in gramicidin A is R-Gly-L-Ala-D-Leu-L-Ala-D-Val-R′. Complete the Fischer projection below by adding the correct group to each vertical bond.arrow_forwardIn a folded protein, Glu116 is close in three-dimensional space to Lys224. The pKa of the carboxylic acid functional group in the side chain of glutamic acid is 4.3. The pKa of the amino group in the side chain of lysine is 10.5. How do you think the pKa of the side chain of Glu116 will change in the folded protein? a. The pKa will be > 4.3. b. The pKa will be < 4.3. c. The pKa will not change. Use chemical reasoning to justify your answer.arrow_forwardIt is estimated that the most common amino acid in a protein is leucine. What is the relative leucine frequency (in %) in the E. coli protein: NP_417192.1 (protein length: 750 aa)? (type only the numeric value two digits after decimal)arrow_forward
- Does Your PDB code: 3GRS have a quaternary structure? YES OR NO? https://www.rcsb.orgarrow_forwardGiven the following DNA sequence: 3'-TACTTNGTNCTNTCN-5' where N stands for any nucleotide, give the complementary mRNA sequence. Indicate direction of strand as 3'--> 5' or 5'--> 3' as in the given sequence above. Give the amino acid sequence of your mRNA sequencelin No. 1. Indicate direction of strand as above. Use all lowercase letters, 3-letter name of amino acid separated by a hyphen (-), no spaces in-between.arrow_forwardThe isoelectric point, pI, of the protein deoxyribonuclease I is 10.2 , while that of pepsin is 1. What is the net charge of deoxyribonuclease I at pH 7.3 ? What is the net charge of pepsin at pH 3.5 ? The isoelectric point of tryptophan is 5.89 ; phenylalanine , 5.48 . During paper electrophoresis at pH 6.5 , toward which clectrode does tryptophan migrate? During paper electrophoresis at pH 4.5, toward which electrode does phenylalanine migrate? [arrow_forward
- You have discovered a novel protein that has a pl = 5.5. To study the functional properties of this new protein, your research group has made a mutant that contains two amino acid changes: a surface phenylalanine residue in the normal protein has been replaced by histidine (side chain pK, = 6.1), and a surface glutamine has been replaced by glutamic acid (side chain pK, = 6.0). Is the pl of the mutant protein predicted to be greater than, less than, or the same as the pI of normal protein? Support your answer with the appropriate calculation.arrow_forwardFor each polypeptide derived in the following sequences: 5' CAA GAG GUA UCC UAC AGA 3' 5' GUC AUC UGG AGG GGC AUU 3' 5' CUA UGC AGU AGG ACA CCC 3' 1. Draw the structure of each polypeptide. 2. Label the amide bonds. 3. Identify the N-terminal and C-terminal amino acids. 4. Write the name of each polypeptide.arrow_forward5 pts Question 9 95 58 40 HS HS SH 65 8 M urea and 72 B-mercaptoethanol 65 Hs84 Hs- SH 72 26 HS 110 SH 84 26 110 95 124 40 58 Native ribonuclease Denatured reduced ribonuclease Figure 2.53 Biochemistry, Seventh Edition O 2012 W. H. Freeman and Company The image above shows how Anfinsen unfolded ribonuclease for his classic experiment to see if proteins contain all necessary information for adopting their folded states on their own. To achieve proper refolding and ribonuclease activity, Anfinsen must have: trick question: ribonuclease cannot adopt its folded state without the help of cellular machinery removed the mercaptoethanol followed by the urea very carefully removed both the urea and mercaptoethanol at the same time removed the urea followed by the mercaptoethanolarrow_forward
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