So we did an experiment and I am curious on the following because the prof did not elaborate too much. She just gave the postive result and reagent. 1. What type of reaction occurred when the samples (enumerated) reacted with the Bial’s reagent? Give the chemical equation for each. a. Glucose b. Sucrose c. Fructose d. Lactose e. Galactose f. Ribose 2. Explain the mechanism behind the test in simple terms. 3. What are the important products of the reaction of each sample?

So we did an experiment and I am curious on the following because the prof did not elaborate too much. She just gave the postive result and reagent. 1. What type of reaction occurred when the samples (enumerated) reacted with the Bial’s reagent? Give the chemical equation for each. a. Glucose b. Sucrose c. Fructose d. Lactose e. Galactose f. Ribose 2. Explain the mechanism behind the test in simple terms. 3. What are the important products of the reaction of each sample?

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter7: Basic Clinical Microbiology

Section7.7: Bacterial Identification And Antibiotic Susceptibility Testing

Problem 3CS

See similar textbooks

Similar questions

A pentapeptide was isolated and upon hydrolysis with various enzymes and chemical test, the following results were obtained. a. Pentapeptide with aminopeptidase releases an amino acid which gives a black precipitate with lead acetate test. b. Tetrapeptide with carboxypeptidase gives a yellow color with ninhydrin test. c. Tripeptide with trypsin releases an amino acid that gives a red color with Sakaguchi test. d. Dipeptide with chymotrypsin separated an amino acid that gives a red color with Millons test e. Amino acid separated is optically inactive. Main questions: A. What amino acids are indicated to be present? B. Deduce the sequence of the amino acid in the pentapeptide.
Lead Acetate Reaction To 1 mL of the sample in a test tube add 1 mL of 40% NaOH. Boil the content for at least 3 minutes. After cooling, add 2 – 3 drops of 10% Pb(OAc)2. Note the color of the solution or precipitate formed. Question 1. What happened to the proteins after adding 40% NaOH and heating the solution?Question 2. What specific amino acid is detected in this reaction?Question 3. What is the identity of the precipitate, if there is any?
"Sugars that give a positive Benedicts test are called reducing sugar." i. Taking glucose as an example, explain in detail the statement above and include all chemical reactions involved. ( ii. Fructose was found to give positive results to Benedict's test even the structure does not reflect the reducing sugar characteristics. Using a diagram, explain what happened to this fructose during the test?
Fructose, sucrose, lactose, xylose, and glucose were tested using Benedict's, Barfoed's, Bial's, and Seliwanoff's tests. Consider a case in which there was no way to heat the samples. Would increasing the concentration of acid in the solution, in lieu of heating, work for the Barfoed's test? Why or why not?
Name the complex formed by the addition of concentrated sulfuric acid to sugar solution and explain thereaction. 2. Why sucrose gives negative Benedict test? 3. Explain, although starch has free hemiacetal bond it gives negative Benedict test. 4. Why glucose (monosaccharide) and maltose (disaccharide) give positive Benedict test?5. What is the difference between Benedict and Barfoed's reaction?6. What are the carbohydrates’ that give positive result with Seliwanoff ? why?
1. What type of reaction occurred when the samples (enumerated) reacted with the Molisch reagent? Give the chemical equation for each.a. Glucoseb. Sucrosec. Fructosed. Lactosee. Galactosef. Ribose 2. Explain the mechanism behind the test in simple terms. 3. What are the important products of the reaction of each sample?
What test reagent is most sensitive in detecting sugar in urine? What are the possible positive results (color) and its interpretation? What test could be used to differentiate: sucrose and lactose? Explain. glucose and starch? Explain. glucose and fructose? Explain. galactose and fructose? Explain An unknown carbohydrate gave a brick red precipitate when tested with Fehling's reagent, turned red when reacted with Seliwanoff's reagent, and quickly gave a red precipitate when reacted with Barfoed's reagent. What conclusions can be made about this carbohydrate? Illustrate with equation the reaction of glucose, galactose, maltose & lactose with osazone test. Sketch the crystals formed for each of the following sugars examined under the microscope.
The following are the sugars that formed brick red precipitate during Barfoed's test, except:* A. GlucoseB. Fructose C. Lactose D. Galactose E. None of the given options
Instead of Molisch's test, what specific test can be used to detect the presence of pentose sugar? Write the procedure
In the acid- catalyzed hydrolysis of starch, how can you tell when the hydrolysis of starch is complete, why does the test work this way? What is the monosaccharide at the end?
Is Raffinose tri-saccharide a reducing sugar? Explain the chemistry of the reducing sugar test and apply it to the tri-saccharide attached.
A. You are given the following carbohydrates: glucose, maltose, sucrose, and starch. Explain the results described below. a. A carbohydrate solution gave a negative result in the Benedict's and Barfoed's tests. When treated with hydrochloric acid and boiled for several minutes, the solution showed a positive result. Explain. b. A solution containing only one carbohydrate gave a Cu2O precipitate with Benedict's reagent. Which possible carbohydrates are present in the solution? c. Another sample of the solution from (b) failed to give a Cu2O precipitate with Barfoed's reagent within 5 min. Which carbohydrate is present in the solution?[