Question #4 The graph on the left shows data for an experiment in which isolated mitochondria were suspended in buffered medium. In the experiment the ability of the mitochondria to consume oxygen (dashed lines) and produce ATP (solid lines) was monitored after the addition of various compounds. Complete the graph to show what happens (how the oxygen consumption and ATP production of the mitochondria change) at steps 1 to 4 in the experiment. In the boxes below briefly explain your answer for each step.

Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter19: The Tricarboxylic Acid Cycle
Section: Chapter Questions
Problem 10P
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Please list steps

Oxygen consumed
Time
1 2 3
STEPS
4A Step 1. ADP+Pi is added
4B Step 2. Succinate is added
4C Step 3. ATP synthase inhibitor
Oligomycin is added.
4D Step 4. The compound FCCP
(Carbonyl-p-
trifluoromethoxyphenylhrdrazone) is
added. FCCP carries protons across the
inner-membrane and dissipates any
proton gradient.
Finish
Experiment
4
EXPLANATION
ATP Synthesized
Question #4
The graph on the left shows data for an
experiment in which isolated
mitochondria were suspended in
buffered medium. In the experiment the
ability of the mitochondria to consume
oxygen (dashed lines) and produce ATP
(solid lines) was monitored after the
addition of various compounds.
Complete the graph to show what
happens (how the oxygen consumption
and ATP production of the mitochondria
change) at steps 1 to 4 in the
experiment. In the boxes below briefly
explain your answer for each step.
Transcribed Image Text:Oxygen consumed Time 1 2 3 STEPS 4A Step 1. ADP+Pi is added 4B Step 2. Succinate is added 4C Step 3. ATP synthase inhibitor Oligomycin is added. 4D Step 4. The compound FCCP (Carbonyl-p- trifluoromethoxyphenylhrdrazone) is added. FCCP carries protons across the inner-membrane and dissipates any proton gradient. Finish Experiment 4 EXPLANATION ATP Synthesized Question #4 The graph on the left shows data for an experiment in which isolated mitochondria were suspended in buffered medium. In the experiment the ability of the mitochondria to consume oxygen (dashed lines) and produce ATP (solid lines) was monitored after the addition of various compounds. Complete the graph to show what happens (how the oxygen consumption and ATP production of the mitochondria change) at steps 1 to 4 in the experiment. In the boxes below briefly explain your answer for each step.
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