(a) The kinetic data given below are for the reaction catalyzed by prostaglandin endoperoxide synthase. Focusing here on the first two columns, determine the Vmax and Km of the enzyme. [Arachidonic acid] Rate of formation of PGG₂ (MM) (mm/min) 0.5 1.0 1.5 2.5 3.5 23.5 32.2 36.9 41.8 44.0 Rate of formation of PGG₂ with 10 mg/mL ibuprofen (mm/min) 16.67 25.25 30.49 37.04 38.91 (b) Ibuprofen is an inhibitor of prostaglandin endoperoxide synthase. By inhibiting the synthesis of prostaglandins, ibuprofen reduces inflammation and pain. Using the data in the first and third columns of the table, determine the type of inhibition that ibuprofen exerts on prostaglandin endoperoxide synthase using a Lineweaver-Burke PLOT.
(a) The kinetic data given below are for the reaction catalyzed by prostaglandin endoperoxide synthase. Focusing here on the first two columns, determine the Vmax and Km of the enzyme. [Arachidonic acid] Rate of formation of PGG₂ (MM) (mm/min) 0.5 1.0 1.5 2.5 3.5 23.5 32.2 36.9 41.8 44.0 Rate of formation of PGG₂ with 10 mg/mL ibuprofen (mm/min) 16.67 25.25 30.49 37.04 38.91 (b) Ibuprofen is an inhibitor of prostaglandin endoperoxide synthase. By inhibiting the synthesis of prostaglandins, ibuprofen reduces inflammation and pain. Using the data in the first and third columns of the table, determine the type of inhibition that ibuprofen exerts on prostaglandin endoperoxide synthase using a Lineweaver-Burke PLOT.
Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter25: Nitrogen Acquisition And Amino Acid Metabolism
Section: Chapter Questions
Problem 12P
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