Observations and Resultsml ofDilutionPlatedFinalDilutionon PlateNumberofColoniesBacterialCount per mlof Sample(CFU/ml)AverageCount per mlof Sample(CFU/ml)DilutionFactorPlate1A H|1:10Iml18 :100 ImlIml28 H41:10000Iml3A H5:100.000iml1010100100010310000104100000 1052A H31:10003Btable helpbfor the missing boxes

Introduction The Stepwise Dilution Of A Substance In Solution Is Referred To As Serial Dilution.…

Observations and Results Average Count per ml of Sample (CFU/ml) Bacterial Dilution Factor ml of Dilution Plated Final Dilution on Plate Number of Colonies Count per ml of Sample (CFU/ml) Plate Iml 10 10 18 21:100 Iml 2A #3:1000 iml 28 H4:10000 Iml 3A H5:100.000 iml 100 10° 1000 103 10000. 104 100000 10 3B

Observations and Results Average Count per ml of Sample (CFU/ml) Bacterial Dilution Factor ml of Dilution Plated Final Dilution on Plate Number of Colonies Count per ml of Sample (CFU/ml) Plate Iml 10 10 18 21:100 Iml 2A #3:1000 iml 28 H4:10000 Iml 3A H5:100.000 iml 100 10° 1000 103 10000. 104 100000 10 3B

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter9: Parenteral Medication Labels And Dosage Calculation

Section: Chapter Questions

Problem 1.5P

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A PhD student leaving for vacation has asked an undergraduate student to perform daily media changes forhis iPSCs while he is gone. The culture is happening in 12-well plates where a volume of 2 mL is optimal. OnSaturday, when changing the media, the undergraduate decides to add 4 ml of media to the dishes (insteadof 2 ml) because he wants to skip lab and watch the Super Bowl on Sunday. He decides to add twice thevolume of media (4 mL) to tide the cells over till Monday. However, when the graduate student returns onMonday, he finds that some of his cells have died. Your job is to determine whether the cells died due to alack of oxygen. For the calculations that follow, diffusion and reaction occurs only in one direction. Also,assume that reaction only occurs at the cell-media interface. Use Michaelis-Menten type kinetics for oxygenuptake. You may use the following information:Ps = 150 mmHg (ambient oxygen tension)K = 1.19 nmol / mL / mmHg (solubility of oxygen in medium)D = 2…
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