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procedure/s in performing aseptic transfer of bacterial cultures in (include illustration)
(3) agar plate to agar slant.
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Solved in 3 steps
- procedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (2) agar slant culture to agar slantprocedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (1) broth culture to brothIn performing the Kirby-Bauer procedure in a clinical laboratory setting, why must the agar be a certain depth? Why must the absorbance of the inoculum be standardized?
- In aseptic technique, what will happen if the inoculated solid plated media is not inverted prior to incubation?Why should agar media be completely dissolved before they are dispensed in tubes and plates? What are the bases for pegging the temperature at 1210C for 15-30 minutes during moist heat sterilization and 1800C for two (2) hours using dry heat sterilization? Can you sterilize culture media using dry heat sterilization? Why is that so? You will notice in the videos shown, the cotton plug is not used. What is role of cotton plug in media prep, sterilization and culture of microorganisms? Instead of using cotton plug, plastic screw-cap is used, can you substitute this for the former? Is it technically acceptable in microbiology?You are tasked to observe the motility of an UNKNOWN Bacteria isolated from a mixed culture, which technique/s is BEST to perform? How shall the procedure be carried out? Explain.
- during inoculation, the bacterial culture tube is always held at an angle and the lid of the Petri dish is slightly open. Explain the purpose of these steps briefly.Blood agar is often used to observe changes in the appear-ance of the agar around the colonies growing on this medium.This medium could then be called:(a) Selective(b) Designated(c) Differential(d) Defined(e) ExactWhat is the physical appearance (solid, liquid, semi-solid) of following media:Agar deep tube
- What is the physical appearance (solid, liquid, semi-solid) of following media: agar plateThe number of bacterial cells in a culture broth is to be determined by a culture technique. Serial dilutions were performed and a 0.1 mL aliquot from each dilution was spread onto Plate Count Agar (PCA). The number of bacterial colony forming units (CFU) after overnight incubation are shown as listed in the table below. What is the number of colony forming units per mL of the culture broth? Choose only the most appropriate plate for your calculation. Give your answer as the number only (do not add text for the units). You may use scientific notation with the format 1.12e+6 (that is, 1.12 x 106 cfu/mL). (Note: Canvas will then display your answer a whole number.) Plate 1 10 Plate 2 10 Plate 3 10 dilution dilution dilution Plate 4 10 dilution Plate 5 107 dilution Plate 6 10 dilution -6 *Too many to count Number of colony forming units (CFU) TMTC* TMTC* 840 28 19 1a. Explain whether or not any of the methods in fi gure 2.9 could beused to determine the total number of cells present in a patient’s specimen.b. After performing the streak plate method on a bacterial specimen, theculture was incubated for 48 hours at 37°C. Upon viewing the plate, therewas heavy growth (with no isolated colonies) in the fi rst quadrant, but nogrowth was apparent in the remaining quadrants. Please discuss errors in the procedure that could have produced this result.
