Spread-plate method Solid agar Incubate Culture, diluted as needed Bacterial colonies 0.1-0.2 mL appear only on surface. Spread cells onto surface of pre-poured solid agar. Pour-plate method 0.1-1.0 mL Melted cooled agar Incubate FIGURE 4.18 Spread Plates and Pour Plates Some colonies appear on surface; many are below surface. These techniques can be used for plate counts. Add melted cooled agar and swirl gently to mix.
Spread-plate method Solid agar Incubate Culture, diluted as needed Bacterial colonies 0.1-0.2 mL appear only on surface. Spread cells onto surface of pre-poured solid agar. Pour-plate method 0.1-1.0 mL Melted cooled agar Incubate FIGURE 4.18 Spread Plates and Pour Plates Some colonies appear on surface; many are below surface. These techniques can be used for plate counts. Add melted cooled agar and swirl gently to mix.
Chapter10: Reconstitution Of Powdered Drugs
Section: Chapter Questions
Problem 17SST
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Why are the results of plate counts expressed as the number of colony-forming units, rather than the number of cells?
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