Principles of Biology
Principles of Biology
2nd Edition
ISBN: 9781259875120
Author: Robert Brooker, Eric P. Widmaier Dr., Linda Graham Dr. Ph.D., Peter Stiling Dr. Ph.D.
Publisher: McGraw-Hill Education
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Chapter 9.5, Problem 1CC
Summary Introduction

To determine:

Whether the leading or lagging strand is made discontinuously in the direction opposite the movement of the replication fork.

Introduction Principles of Biology, Chapter 9.5, Problem 1CC

DNA replication is semiconservative mechanism because, at the end of the replication cycle, each DNA molecule has one complete strand from the parent or original DNA molecule. Different replication enzymes such as DNA helicase, DNA polymerase, and DNA ligase helps in the process of replication.

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Why can’t the lagging strand be synthesized in the same direction of the replication fork?
If deoxyribonucleotides that lack the 3’-OH groups are added during the replication process, what do you expect will occur?
What is the difference between the leading strand and the lagging strand in DNA replication?     There are different DNA polymerases involved in elongation of the leading strand and the lagging strand.     The leading strand is synthesized continuously in the 5' → 3' direction, while the lagging strand is synthesized discontinuously in the 5' → 3' direction.     The leading strand requires an RNA primer, whereas the lagging strand does not.     The leading strand is synthesized in the 3' → 5' direction in a discontinuous fashion, while the lagging strand is synthesized in the 5' → 3' direction in a continuous fashion.
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