Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
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Textbook Question
Chapter 6, Problem 3A
Most laboratory media contain a fermentable carbohydrate and peptone because the majority of bacteria require carbon, nitrogen, and energy sources in these forms. How are these three needs met by glucose-minimal salts medium? (Hint: See Table 6.2.)
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Yeast cells are added to a 3.0 L batch bioreactor so that the initial cell concentration is [X]. = 1.3 g cells / L.
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Menaquinones is produced by Flavobacterium meningosepticum via submerged fermentation with 60% working volume in 30-L fermentation. To obtain the crude menaquinones, the fermentation medium is centrifuged at 12,000 rpm for 15 minutes. The vacuum-freeze dried particles are crushed and collected. After that the samples are treated with methanol for 20 minutes. To remove polar lipid compounds from bacterial cells, HZ816 macroporous adsorption resin column is used with methanol-dichloromethane (l:1) as mobile phase and flow rate of 0.75 mL/min. Rotary evaporation at 500 C is carried out to concentrate the eluent. After that, the concentrated sample is purified with Bio-BeadsTM S-X3 to remove components without UV absorption. To obtain the homolog purified menaquinones, RP-C18 column is used. Recrystallization is carried out to obtain pure crystalized menaquinones.
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Sydney Brenner isolated Salmonella typhimurium mutants that were
implicated in the biosynthesis of tryptophan and would not grow on
minimal medium. When these bacterial mutants were tested on minimal
medium to which one of four compounds (indole glycerol phosphate,
indole, anthranilic acid, and tryptophan) had been added, the growth
responses shown in the following table were obtained.
Mutant
Minimal
medium
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acid
Indole Tryptoph
phosphate
trp-1
trp-2
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trp-4
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trp-11 -
Give the order of indole glycerol phosphate, indole, anthranilic acid, and
tryptophan in a biochemical pathway leading to the synthesis of
tryptophan. Indicate which step in the pathway is affected by each of the
mutations.
+ 1 + 1 II
Chapter 6 Solutions
Microbiology: An Introduction
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