Microbiology: Principles and Explorations
10th Edition
ISBN: 9781119390114
Author: Black
Publisher: WILEY
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Chapter 1, Problem 2.4SC
Why was the French microbiologists’ method of broth dilution inadequate for obtaining pure cultures of organisms?
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Chapter 1 Solutions
Microbiology: Principles and Explorations
Ch. 1 - List three reasons to study microbiology.Ch. 1 - What is the difference between microbiology and...Ch. 1 - Prob. 1.3SCCh. 1 - List five bacterial diseases and five viral...Ch. 1 - Prob. 2.1SCCh. 1 - State the germ theory of disease. Try to think of...Ch. 1 - How did Pasteurs experiment with swan-necked...Ch. 1 - Why was the French microbiologists method of broth...Ch. 1 - What were the scientific contributions of Jenner,...Ch. 1 - Prob. 3.2SC
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- a) explain in your own words how to do a nitrate reduction test to identify unknown bacteria  b) explain in your own words how to perform a phenol red broth (prb) test to help identify unknown bacteriaarrow_forwardTransparent double-sided dishes used for growing microbes are most commonly called A) Petri dishes. B) baker dishes. C) sterilization plates. D) culture medium plates.arrow_forwardWhat are the advantages and disadvantages of culture-dependent and culture-independent methods in microbiology, respectively.arrow_forward
- Why might viable cell cultures be of more use in microbialtaxonomy than preserved specimens?arrow_forwardWhat is the importance of using a test/control organism in describing the cultural characteristics of an unknown bacterium?arrow_forwardYou are given a mix culture of S. aureus,E. coli and P. aeruginosa. Besides the streak plate method what other methods could you use to separate the bacteria? Please state what method(s) you would use, the result you would be looking for to help identify each bacterium and the interpretation of the result.arrow_forward
- The use of sterile techniques and agar media enables microbiologists to study: a) Bacterial growth b) Nutrient agar-metabolizing culture c) Bacterial metabolism d) Pure cultures d) Bacterial life cyclarrow_forwardMary wanted to know the amount of microbes in her garden. To do this, she took a 10g soil sample, added urea, and then let it incubate for one week. She then performed serial dilution a shown below: 10 g soil sample To: 1ml 90 ml Dilution Bottle 1 ml 9 ml Tube 1 0.1 ml 0.45 ml 111 9.9 ml Tube 3 9 ml Tube 2 9.55 ml Tube 4 Calculate the dilution of the bottle and each tube. Be sure to show complete solutions and box your final answer.arrow_forwardWhat is the difference between a mixed culture and a contaminated culture if they both contain more than one type of organism ?arrow_forward
- A student needed to transfer bacteria from a broth culture to an agar plate. Below is the step-by-step what was done to accomplish this. The transfer of bacteria was not successful because of which step? 1. Cap of the broth culture is removed 2. The mouth of the bottle is flamed 3. The loop was flamed 4. The loop was inserted into the culture to pick up the bacteria 5. The loop was flamed 6. The loop containing the bacteria was used to introduced to spread the agar plate 7. The plate was placed in an incubator at 30 Celciusarrow_forward1. A plate with a final dilution factor of 107 produced 210 colonies. a) What was the original concentration in the sample? b) If you were to dilute the original sample with a dilution factor of 108, how many colonies would you count on the plate? c) If you were to dilute the original sample with a dilution factor of 106, how many colonies would you count on the plate?  2. Sally Monella found 344 bacterial colonies on one of her plates. She had prepared this plate after a serial dilution of a culture of Yersinia pestis. She had pipetted 1 ml sample of diluted Yersinia pestis from a tube with the overall dilution factor of 106 into a plate and covered it with agar. She used this plate to calculate the concentration in cells/ml of a bacterial culture. a) How many organisms were in the original culture? b) Were her results valid? Why or why not?arrow_forwardIn the preparation of a bacterial smear, why is there a need to fix the bacteria to the slide? Aside from passing the slide over a flame, what are the other ways of fixing the bacteria to the slide?arrow_forward
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