Becker's World of the Cell (9th Edition)
9th Edition
ISBN: 9780321934925
Author: Jeff Hardin, Gregory Paul Bertoni
Publisher: PEARSON
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Chapter 1, Problem 1.4PS
Problem Set
QUANTITATIVE Limits of Resolution Then and Now. Based on what you learned in this chapter about the limit of resolution of a light microscope, answer each of the following questions. Assume that the unaided human eye has a limit of resolution of about 0.25 mm and that a modern light microscope has a useful magnification of about 1000×.
(a) Define limit of resolution in your own words. What was the limit of resolution of Hooke’s microscope? What about van Leeuwenhoek’s microscope?
(b) What are the approximate dimensions of the smallest structure that Hooke would have been able to observe with his microscope? Would he have been able to see any of the structures shown in Figure 1-3a? If so, which ones? And if not, why not?
(c) What are the approximate dimensions of the smallest structure that van Leeuwenhoek would have been able to observe with his microscope? Would he have been able to see any of the structures shown in Figure 1-3a? If so, which ones? And if not, why not?
(d) What are the approximate dimensions of the smallest structure that a contemporary cell biologist should be able to observe with a modern light microscope?
(e) Consider the eight structures shown in Figure 1-3a and 1-3b. Which of these structures would both Hooke and van Leeuwenhoek have been able to see with their respective microscopes? Which, if any, would van Leeuwenhoek have been able to see that Hooke could not? Explain your reasoning. Which, if any, that neither Hooke nor van Leeuwenhoek could see would a contemporary cell biologist be able to see using a modern light microscope?
Figure 1-3 The Worlds of the Micrometer and Nanometer. Illustrations show (a) typical cells and (b) common cellular structures.
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Sort the following characteristics based on whether they apply to light or electron microscopes.
2000x
1,000,000x or more
200 mm (0.2um)
Yes
No
Usually not
0.5 nm
Table 3.3 Comparison of Light Microscopes and Electron Microscopes
Characteristic
Light or Optical
Electron (Transmission)
Useful magnification
200 mm (0.2µm)
1,000,000x or more
Maximum resolution
200 mm (0.2um)
1,000,000x or more
Image produced by
Light rays
Electron beam
Image focused by
Glass objective lens
Electromagnetic objective lenses
Image viewed through
Glass ocular lens
Fluorescent screen
Specimen placed on
Glass slide
Copper mesh
Specimen may be alive
Yes
Specimen requires special stains or treatment
Depends on technique
Yes
Shows natural color
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QUESTION -
What information can you get from a residuals plot that has a pattern rather than a random appearance? PLEASE EXPLAIN ! THIS IS UV-VIS SPECTRUM
Phase contrast microscopy - Human Cheek Cells
a.
b.
What is the basic principle of image formation using this microscopy technique?
What can be observed and concluded from the image of the specimen?
Chapter 1 Solutions
Becker's World of the Cell (9th Edition)
Ch. 1 - What evidence led scientists to develop the basic...Ch. 1 - Why is a high degree of antibody-antigen...Ch. 1 - You have discovered a defective gene that allows...Ch. 1 - Prob. 1.3CCCh. 1 - The Historical Strands of Cell Biology. For each...Ch. 1 - QUANTITATIVE Cell Sizes. To appreciate the...Ch. 1 - QUANTITATIVE Sizing Things Up. To appreciate the...Ch. 1 - Problem Set QUANTITATIVE Limits of Resolution Then...Ch. 1 - The Contemporary Strands of Cell Biology. For each...Ch. 1 - The Facts of Life. Each of these statements was...
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