You will only isolate pure colony to inoculate again (sub-culturing) to newly prepared plate medium, slant agar tube, and broth tube for further study and identification Question from the picture above (pure colonies white pinhead size) How many pure colonies of microorganisms are in the petri dish sample?
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You will only isolate pure colony to inoculate again (sub-culturing) to newly prepared plate medium, slant agar tube, and broth tube for further study and identification
Question from the picture above (pure colonies white pinhead size)
- How many pure colonies of microorganisms are in the petri dish sample?
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- Describe how you would execute this first stage from the point you are handed the nutrient broth tube containing the mixed culture, through to the appearance of two colony types on a nutrient agar plate. Assume you have all the necessary equipment and materials at your disposal. Be concise, but thorough; limit yourself to a short paragraph (1/4-1/2 page at most) – include the method and techniques; what you expect to see, and how you would avoid contamination during the process.Drawings of the two bacterial cultures as observed under the 4 mm objective. Describe features observable under the high-power objective and their movement as seen in the hanging drop preparationGiven the scenario, compute for the total volume of the culture media solution (milliliter or liter) and dehydrated media (grams). Scenario: The students of a Microbiology class were tasked to transfer or subculture a pure culture of Escherichia coli bacterium in five 7 mL nutrient broth and five petri dishes of nutrient agar with 20 mL capacity each. Based on the instruction bottles for nutrient broth and nutrient agar, preparation of the culture media is as follows. Nutrient broth: 8 g/liter Nutrient agar: 28 g/liter Formula: C1V1 = C2V2 *Concentration *Volume Computation: What are the answers to the following. Weight in grams of nutrient broth: _________ Distilled water in mL for nutrient broth: __________ Weight in grams of nutrient agar __________ Distilled water in mL for nutrient agar: ____________
- Technique used to inoculate plate media using inoculating loop. Technique used to inoculate agar deep using inoculating needle. Pattern used to inoculate agar slant to get luxuriant culture. This pattern increases the surface area of agar that can be inoculated. Can you answer all the questions? Thankyou!Create a graph of this spectrophotometric data (using Excel or the graph paper below), labeling the four stages of the growth curve- lag, log (exponential), stationary, death. Be sure to label your axes properly and give your graph a title Time Culture Sampled % Absorbance 0 min 5% 30min 7% 60min 9% 90min 15% 120min 25% 150min 37% 180min 49% 210min 50% 240min 49% 270min…Subculture and Colony Morphology Descriptions Following identification of the Gram-negative isolate and Gram-positive isolate, you next subculture each onto fresh nutrient agar plates. Briefly describe the subculture process in three or so sentences and what this allowed you to achieve; follow this with colony morphology descriptions of each. Description: Isolate A – Describe: Colony morphology: Medium & incubation temperature: Isolate B – Describe: Colony morphology: Medium & incubation temperature:
- In a bacterial culture on an agar plate, what is a zone of inhibition? An area where the growth of bacteria is increased compared to the rest of the agar plate An area where the growth of bacteria is prevented or reduced compared to the rest of the agar plate An area where the growth of bacteria is not affected compared to the rest of the agar plate An area where the the agar becomes contaminated with moldUpon observation of the nutrient agar slant culture, you strongly suspect that the culture is contaminated. Outline the method you would follow to ascertain whether your suspicion is justified.Can you please discuss the principles of using Motility Test Medium for the detection of bacterial motility. Focus on the components of the medium and the purpose of each component. Note: You may refer to different culture media manuals like Difco, BBL, etc.
- A student needed to transfer bacteria from a broth culture to an agar plate. Below is the step-by-step what was done to accomplish this. The transfer of bacteria was not successful because of which step? 1. Cap of the broth culture is removed 2. The mouth of the bottle is flamed 3. The loop was flamed 4. The loop was inserted into the culture to pick up the bacteria 5. The loop was flamed 6. The loop containing the bacteria was used to introduced to spread the agar plate 7. The plate was placed in an incubator at 30 CelciusWhy is it important to use a sterilized loop between streaks when preparing a streakplate? Observation of a streakplate culture shows more growth in Quadrant 4 than in Quadrant 3. Account for this observation. Describe the way in which you can isolate an individual colony from a spreadplate or a streakplate that holds multiple colonies. Outline the differences between a streak plate and a spread plate.You are rotating sterile supplies based on the first in, first out (FIFO) principle. What would you look at? O Sterilization method Expiration date Manufacturing date Packaging material