What is the purpose of flaming an inoculating loop? How will you know when you have flamed the loop or needle long enough? Why is it necessary to cool the inoculating loop prior to obtaining the bacterial sample? In which direction should you move the inoculating loop in the Bunsen burner flame? (i.e. from the handle to the loop or from the loop to the handle)

What is the purpose of flaming an inoculating loop? How will you know when you have flamed the loop or needle long enough? Why is it necessary to cool the inoculating loop prior to obtaining the bacterial sample? In which direction should you move the inoculating loop in the Bunsen burner flame? (i.e. from the handle to the loop or from the loop to the handle)

Microbiology for Surgical Technologists (MindTap Course List)

2nd Edition

ISBN:9781111306663

Author:Margaret Rodriguez, Paul Price

Publisher:Margaret Rodriguez, Paul Price

Chapter4: The Prokaryotes

Section: Chapter Questions

Problem 3UTM: A surgical technologist working in the Central Sterile Processing Department is asked to run the...

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A surgical technologist working in the Central Sterile Processing Department is asked to run the steam sterilizer (autoclave) with a biological monitor for the first load of the day without any instrument trays or items. What must be done with the bacterial sample after it is processed in the sterilizer?
In the preparation of a bacterial smear, why is there a need to fix the bacteria to the slide? Aside from passing the slide over a flame, what are the other ways of fixing the bacteria to the slide?
Would you use an inoculating loop to transfer bacteria to an agar deep tube? Would you use an inoculating loop to transfer bacteria to an agar deep tube? Yes, as long as you insert the loop into the agar smoothly. No, a needle would be best since it will do the least amount of damage to the agar and allow it to reseal around the inserted bacteria. It depends on which bacteria you are transferring. Yes, as long as you are careful when streaking on the surface of the agar.
during inoculation, the bacterial culture tube is always held at an angle and the lid of the Petri dish is slightly open. Explain the purpose of these steps briefly.
In aseptic technique, What will/may happen if you open a solid plate and not pass the inoculating loop or inoculating needle over a flame to take a colony or when you are about to inoculate on it?
A patient sample will be analyzed. You are responsible to quantify the bacteria number. The dilution series shown in the Figure ( Dilution.jpg) is prepared and 1 ml from each tube is plated. Plates were grown 24 hours resulting in colonies in each plate. Which plate you will use to quantify the bacteria in the patient sample? Why did you pick that plate? What is the dilution factor used in this series? Calculate the number of bacteria in the original patient sample. What would you do and why, if there were too many colonies to count on each plate?
A student needed to transfer bacteria from a broth culture to an agar plate. Below is the step-by-step what was done to accomplish this. The transfer of bacteria was not successful because of which step? 1. Cap of the broth culture is removed 2. The mouth of the bottle is flamed 3. The loop was flamed 4. The loop was inserted into the culture to pick up the bacteria 5. The loop was flamed 6. The loop containing the bacteria was used to introduced to spread the agar plate 7. The plate was placed in an incubator at 30 Celcius
Why is a Bunsen burner used in the microbiology lab? (select all that apply) The flame is used to sterilize the inoculating loop. The flame kills microorganisms that float in the air and could contminate samples. To sterilize the lid of the agar plate. To reduce the spread of antibiotic resistance.
How many different types of colonies can you find on the blood agar plate? describe each. (As shown in pictures below) What possible types of bacteria ? (As shown in pictures below)
When testing the efficacy of an antibiotic against bacteria on an agar plate, it is important to spread the bacteria evenly across the plate before you add the antibiotic. Why do the bacteria need to be applied evenly? None of the following are true All of the following are true If you add more bacteria in some areas compared to others, it might obscure the effect of the antibiotic If the area right next to the antibiotic initially received relatively few bacterial cells, that might lead you to overestimate the effectiveness of the antibiotic If the area right next to the antibiotic initially received a relatively large number of bacterial cells, that might lead you to underestimate the effectiveness of the antibiotic
Answer the following questions: 1. Why do we pass the smear in the open flame of the bunsen burner 3 times? What do we want to achieve by doing this? 2. Is the size of the bacterial smear on the slide very critical to the analysis? Why or why not? 3. Is the thickness of the bacterial smear on the slide very critical to the analysis? Why or why not? 4. Why do we pass the smear in a gentle stream of water after flooding it with a stain? What do we want to prevent by doing this? 5. Is the duration or time of stain application very critical in simple staining? Why or why not? NOTE: Please try to answer all of the questions asked, i promise to give you a good ratings
What is the physical appearance (solid, liquid, semi-solid) of following media:Agar deep tube