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Technique used to inoculate plate media using inoculating loop.
Technique used to inoculate agar deep using inoculating needle.
Pattern used to inoculate agar slant to get luxuriant culture. This pattern increases the surface area of agar that can be inoculated.
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- In a bacterial culture on an agar plate, what is a zone of inhibition? An area where the growth of bacteria is increased compared to the rest of the agar plate An area where the growth of bacteria is prevented or reduced compared to the rest of the agar plate An area where the growth of bacteria is not affected compared to the rest of the agar plate An area where the the agar becomes contaminated with moldA student needed to transfer bacteria from a broth culture to an agar plate. Below is the step-by-step what was done to accomplish this. The transfer of bacteria was not successful because of which step? 1. Cap of the broth culture is removed 2. The mouth of the bottle is flamed 3. The loop was flamed 4. The loop was inserted into the culture to pick up the bacteria 5. The loop was flamed 6. The loop containing the bacteria was used to introduced to spread the agar plate 7. The plate was placed in an incubator at 30 CelciusWhat are the primary uses of the following preparations of culture media? Agar regular slant Agar special slant Agar deep Broth Agar plate
- In spread plate method, colonies form within the agar and agar surface. In pour plate method, previously prepared agar plates are not required. a. First statement is TRUE, Second statement is FALSE. b. First statement is FALSE, Second statement is TRUE. c. Both statements are TRUE. d. Both statements are FALSE.Lid of petri dish was left on bench top for a few minutes after pouring of sterile medium. Effect: Rationale:Why go to the trouble of creating a master plate (why not simply plate the initial culture on both nutrient agar and glucose-salts agar)?
- Which of the following is the proper technique for inoculating an agar slant with a broth culture? A. Stab the butt of the media with the wire loop. B. Stab the surface of the agar media with the wire loop beginning at the base of the tube moving toward the mouth as you withdraw the loop. C. Gently move the wire loop back and forth across the surface of the agar beginning at the mouth of the tube moving down toward the base of the slant. D. Gently move the wire loop back and forth across the surface of the agar beginning at the base of the slant as you withdraw it from the tube.What is the purpose of fixing a smear? Mark all that apply: 1. To attach the bacteria to the slide 2. To cause the cells to shrink and become distorted 3. To kill the bacteria so they aren't harmed by the staining method 4. To break down the cell wall in order to make the cells accept stain 5. To kill the bacteria to make the slide safer to handleHow do you sterilize the following? petri dish inoculating loop culture media test tube with culture media beakers and other glassware
- The following data were obtained from pour plates used to test the effectiveness of a food preservative. Two samples of cottage cheese were inoculated with bacteria; the preservative was added to one sample. After incubation, samples of the cottage cheese (control) and samples treated with the preservative (experimental) were diluted and plated on nutrient agar. Calculate the number of bacteria. Was the preservative effective? Dilution Amount Plated (mL) Number of Colonies CFU/mL Control 1:400 1 160 Experimental 1:200 0.1 32When viable cell concentrations are too high to count on an agar plate, it is common to use larger sized plates to increase the surface area for counting the colonies.True or falseYou will only isolate pure colony to inoculate again (sub-culturing) to newly prepared plate medium, slant agar tube, and broth tube for further study and identification Question from the picture above (pure colonies white pinhead size) How many pure colonies of microorganisms are in the petri dish sample?