What is the importance of using an immersion oil in 100x magnification? Describe the changes in the field of view and the amount of available light when going from low to high power using the compound microscope. Give some limitations in the use of microscope.
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- What is the importance of using an immersion oil in 100x magnification?
- Describe the changes in the field of view and the amount of available light when going from low to high power using the compound microscope.
- Give some limitations in the use of microscope.
- Conclusion about microscope
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- Total magnification of a microscope is determined by Multiple Choice dividing the objective magnification value by the ocular value. multiplying all objective magnification values together. adding all objective magnification values together. none of the above.Differentiate between the limit of resolution of the typical light microscope and that of the unaided human eyes the relationship between the depth of field and magnification is that When magnification increases, the depth of field decreases. Name two procedures (steps) you should do to achieve the maximum resolution when using the oil immersion lens State the relationship between the working distance of an objective lens and its magnification power “The coarse focus knob can be used to adjust the focus when using any of the objective lenses”. Is this statement true or false? If it is false, please correct it. When using the oil immersion lens but you cannot focus, what should you should not do? And what you should do? What does this statement mean: “Your microscope is parfocal”? What is the significance of condenser lens? Multiple choice question: The most useful adjustment for increasing image contrast in low power magnification is _______ Closing down the diaphragm Closing one…Previous question: For example, you can estimate the field of view diameter at 400x total magnification using the measured diameter at 100x total magnification. The magnification is 4 times greater so the field of view will be 4 times smaller. What is the field of view under the 40X objective of the microscope. - The Answer I got for this question was 3/4mm.
- Explain when to use bright-field, phase-contrast, dark-field, fluorescence, transmission electron, and scanning electron microscopy for a given situation. What is an example of this situation?Which of the following is true about the relationship between depth of field and magnification? There is no relationship. When magnification increases, depth of field decreases. When magnification increases, depth of field increases. Stereomicroscopes offer a lesser depth of field than compound microscopes. The depth of field of a microscope determines how much magnification it can achieve.What is the purpose of using microscopes? If you need to move the microscope, describe how you should carry it. What three things change as you increase magnification? On the compound microscope, what power must be in place when you first find an image in themicroscope? On the compound microscope, which adjustment knob is used for bringing the specimen intofocus? On the compound microscope, which adjustment knob is used for sharpening the image of thespecimen after it is focused? On the compound microscope, which adjustment knob should you use on the high power? On the compound microscope, how do you change the objective lenses? What should be included when sketching or drawing what is seen through the microscope How do you properly use a microscope?
- What description best describes the compound light microscope? Uses a beam of electrons is transmitted through a specimen to form an image. Has more than one lens and its own light source. In this type of microscope, there are ocular lenses in the binocular eyepieces and objective lenses in a rotating nosepiece closer to the specimen. An optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. Designed for low magnification observation of a sample, typically using light reflected from the surface of an object rather than transmitted through it.Using the microscope Answer the following questions as you work through this exercise: 1. How is the letter “e” on the slide oriented when you see it with the naked eye as you mount it on the stage (i.e., is it right side up or upside down)? 2. How is the letter “e” on the slide oriented when you see it under low or high power magnification? 3. What effect, if any, does the compound light microscope have on the orientation of the image?Please answer the following questions (Question 1 AND 2) about compound microscopes. After clicking the objective into position, which adjustment knob(s) can you use to safely refocus the image? You may need to adjust the light intensity to better see the specimen. Indicate two ways in which you can change the light intensity?
- The following questions talk about increasing magnification in microscopes. Please answer questions 1 and 2 After a specimen is in focus, where in the field of view should position the specimen before increasing the magnification (be precise)? (QUESTION 2 HAS TWO PARTS TO IT): To increase the magnification, which objective should you click into position next? After refocusing the specimen with the new objective, if you want to increase the magnification even more, which objective should you now click into position?An object found with the 10x objective of the microscope may be viewed with the 40x objective without having to re-focus. This property of the microscope is called: O Confocal O Bifocal Binocular d ParfocalComplete Table 1 below by supplying the characteristics of each objective. Table 1. Numerical characteristics of microscope objectives. LPO НРО OIO Focal length (mm) Working distance (mm) Linear magnification (X) Numerical aperture (N.A..) Features (color of the band, diameter of the opening of the lens, etc.)