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- Describe 3 simple ways of preparing slides for light microscopy microscopeDiscussion: 1) What does calibrating the microscope mean? Why do we calibrate the microscope? 2) What is the ocular calibration scale for each objective? Why is important to calibrate each objective?1. Match: Read about each microscope part. Match the description to the part on the diagram. Stage: Platform where a slide is placed. Eye piece: Lens at the top of the microscope that the user looks through. This lens most commonly magnifies a sample by 10x. Coarse focus knob: Large knob that moves the stage up and down to focus the sample. Fine focus knob: Small knob that moves the stage over a short distance to refine the focus. Objective lens: A second lens that further magnifies the sample. Microscopes usually have several objective lenses with different magnifications. Slide: A rectanqular piece of glass upon which a sample is mounted for viewing under a microscope.
- Compare and contrast the operation of optical microscopy and TEM in terms of Contrast (explain all different types for each microscopy) Note: Wherever possible, explain what factors affect or control each features.What are the advantages of using super-resolution microscopyversus standard fluorescence microscopy?3D dimensionality is a limitation of the compound microscope. Depth of field, DOF, describes dimensionality form top to bottom and can be observed with colored cross threads. Observe the crossed thread slide on low power (4x), then on medium power (10x), then on high power (40x objective magnification). Which crossed fiber is on top? How do you know?
- How will the following affect resolution during microscopy? I) Closing or opening the diaphragm II) Raising or lowering the condenser III) Increasing or reducing the light intensityFor each type of microscopy, know how each is used and the type of image produced. kEEP IT SHORT AND SWEET Light microscope – Transmission electron microscope - Scanning electron microscope –Explain when to use bright-field, phase-contrast, dark-field, fluorescence, transmission electron, and scanning electron microscopy for a given situation. What is an example of this situation?
- What advantage do fluorescent dyes and fluorescence microscopy provide in comparison to the chemical dyes used to stain specimens for light microscopy?What are the differences between phase contrast microscopy and differential interference microscopy?List two ways that a stereoscopic dissecting microscope differs from a compound microscope