Explain when to use bright-field, phase-contrast, dark-field, fluorescence, transmission electron, and scanning electron microscopy for a given situation. What is an example of this situation?

Q: Explain how to correctly use the microscope using oil immersion.

A: Light tends to bend while entering one material from a different one. The refraction of light…

Q: Compare and contrast how dark-field, phase-contrast, differential interference contrast,…

A: The technique by which the smaller or minute particles are magnified for studying the features is…

Q: Table 1 - Characteristics of the microscope Low Power (Dry) High Power (Immersion Oil) Features…

A: A microscope is a device that allows you to examine tiny objects, including cells. The picture of an…

Q: Differentiate between the limit of resolution of the typical light microscope and that of the…

A: The microscope is an instrument that helps us to visualize the objects that cannot be seen by our…

Q: If 5X instead of 10X oculars were used with the same objectives now on your microscope, what…

A: Microscopy means the use of microscope for studying the cells. Microscope is an indispensable…

Q: Compare bright-field, dark-field, phase-contrast, confocal, andfluorescence microscopy as to field…

A: Optical microscopes use light source that consists of a band of colored wavelengths in the visible…

Q: What particular features of fluorescence make it useful for microscopy?

A: Step 1 Microscopes are an instrument which is used for observing small objects and finer internal…

Q: What are the main characteristics of scanning electron microscopy?

A: Electrons are the negatively charged particles having minus 1 charge  and represented by e- ,…

Q: Would phase microscopy be of any particular value when examining a stained specimen? Explain

A: The area or field of study in which microscopes are used to view an object that cannot be seen…

Q: Explain why resolving power is important in microscopy.

A: Microscopy can be defined as the technical field which is used to see the objects which are not…

Q: Why is CSLM able to view different layers in a thick preparationwhile bright-field microscopy…

A: The technical field that uses microscopes to observe objects and their areas that cannot be seen…

Q: Explain differential-interference contrast (DIC) microscopy.

A: Microscopy is a technique through which the microscopic objects which cannot be seen through naked…

Q: Compare and contrast light microscopy and electron microscopy

A: Microscopy is the technical field of using microscopes to view objects and areas of objects that…

Q: discuss the microscopy technique that gives the best constrast image of non biological samples such…

A: Microscopy is an instrument used to magnify the size of the objects or particle which cannot be seen…

Q: Compare the appearance of microorganisms you observed using phase-contrast microscopy versus…

A: Microscopy is a basic form of microscopic technology. It depends on the interaction between light…

Q: List two ways that a stereoscopic dissecting microscope differs from a compound microscope

A: The stereoscopic dissecting microscope is often used to study the surface of specimens. The compound…

Q: riefly describe the functions of the following parts of the microscope: course objective knob,…

A: Microscopy is the technique of using a microscope and it demands an understanding of its working and…

Q: What limitation applies to most forms of electron microscopy?

A: When we use Electron microscopy then we face certain limitations or drawbacks. Here we will discuss…

Q: why is microtome important in microscopy?

A: Objects or organisms that cannot be viewed by the unaided eyes are studied under the microscope.…

Q: Between wet mount and hanging drop slide preparations, which is more resistant to evaporation? Which…

A: Bacteria are member of a large group of unicellular microorganisms. It has cell walls but lacks…

Q: An object found with the 10x objective of the microscope may be viewed with the 40x objective…

A: A compound microscope has multiple lenses of different magnification.

Q: Why don’t most light microscopes use 303 ocular lenses for greater magnification?

A: The ‘human eye’ is the most valuable and sensitive sense organ. It is a natural optical instrument.…

Q: What are the advantages of using super-resolution microscopyversus standard fluorescence microscopy?

A: Fluorescence microscope is a type of microscope that makes use of phosphorescence and fluorescence…

Q: Describe the process of bringing a specimen into focus using a compound light microscope.

A: A microscope is an instrument that can magnify specimen which is otherwise too small to be seen,…

Q: The diffraction limit is an unbeatable barrier limiting the effective resolution of light microscopy…

A: Every lens has an absolute upper performance limit dictated by laws of physics, called the…

Q: Why is it important to center your object before moving to higher magnifications in a light…

A: A microscope is a crucial tool in the study of organisms that are very minute or to study the cells…

Q: What is the advantage of phase-contrast microscopyover bright-field microscopy?

A: Phase-contrast microscopy is an optical microscopy technique that converts phase shifts in light…

Q: Provide an illustration and brief description of the pathway of light in relation to magnification…

A: Introduction :- A microscope is a device that magnifies images of small objects, allowing the…

Q: Which of the following correctly traces the path of light through the compound microscope?

A: The correct sequence of the passage of light through a compound microscope is Illuminator(light…

Q: Explain the differences between Brightfield, Darkfield, and Phase-Contrast Microscopy.

A: * Here are some differences between bright field microscopy darkfield miccroscopy and phase contrast…

Q: Explain in detail simple and differential staining and also improving and adjusting contrast in…

A: Staining is a chemical method which is used to colour the organism or the cell so that it is viewed…

Q: How many types of objective lenses a light microscope has?

A: In a microscope, the objective lens lies very close to the specimen. The objective lens collects the…

Q: Describe the use of fluorescent dyes in microscopy.

A: Microscopy is a technical field that involves utilizing microscopes to examine materials and things…

Q: What is the importance of resolution, magnification and contrast in microscopy

A: The lenses of the microscopes create two types of images when a specimen is placed beneath the…

Q: How is confocal scanning laser microscopy differentfrom fluorescence microscopy? In what ways are…

A: Microscope is an analytical instrument which is used to analyse, study and visualize internal…

Q: Why is methylene blue used to stain cells? What does it bind to?

A: Answer: Methyl blue is used to stain the cell because it is basic dye which can easily bind with…

Q: Transmission and scanning electron microscopy differ because transmission electron microscopy shows…

A: As per the guidelines, we are supposed to answer only one question. Kindly repost the other question…

Q: Explain briefly why the 100X objective lens of the light microscope is immersed in  oil.

A: The ability of a microscope to reveal an image that is numerous times larger in size than the…

Q: Stereomicroscopes Bright field microscope Dark field microscope Fluorescence microscope Phase…

A: Microscope is used to view the very fine details of the specimen. these may be stained or unstained…

Q: microscopy, why are different stains used for different samples? Be specific.

A: A microscope is an instrument which is used to observe microscopic specimens which are otherwise not…

Q: Describe 3 simple ways of preparing slides for light microscopy microscope

A: Introduction The optical microscope, often known as a light microscope, is a type of microscope that…

Q: What is the principle of image formation on transmission election microscope and scanning electron…

A: The general biological microscope helps in the visualisation of the image of a particular sample…

Q: Why would specimens viewed with a compound microscope be thin and/or chemically cleared?

A: The human eye can see objects upto 0.1 millimeters. If the objects are smaller than this, the human…

Q: What is the function of staining in light microscopy?

A: The light microscopy or light microscope represents a laboratory instrument required for the…

Q: Discuss the microscopy technique that gives the best contrast image of non-biological samples such…

A: Scanning electron microscopy (SEM) SEM is considered as one of the most popular technique used for…

Q: How do stains used for light microscopy compare withthose used for electron microscopy?

A: The micro-organism is the organism that is too small to be seen with the naked eye. It is viewed by…

Q: Always begin examing microscope slides with which power objective?

A: A microscope is an instrument of immense importance in a biology/ microbiology laboratory. As the…