The calcium ion binds to a certain protein to form a 1:1 complex. The following data were obtained in an experiment. Total Ca2+/µM 60.9 122 186 250. 536 Ca2+ bound to Protein/µM 31.4 51.4 63.6 71.0 83.6 Determine graphically the dissociation constant of the Ca2+-protein complex. The protein concentration was kept at 97 µM for each run. (1 µM = 1x10-6 M.) Kd =
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- The addition of a small amount of a salt, such as (NH4)2SO4, to a solution containing a charged protein increases the solubility of the protein in water. This observation is called the salting-in effect. However, the addition of large amounts of salt can decrease the solubility of the protein to such an extent that the protein precipitates from solution. This observation is called the salting-out effect and is used widely by biochemists to isolate and purify proteins. Consider the equilibrium PXν(s) ⇋ Pν+(aq) + ν X−(aq), where Pν+ is a polycationic protein of charge ν+ and X− is its counterion. Use Le Chatelier’s principle and the physical principles behind the Debye–Hückel theory to provide a molecular interpretation for the salting-in and salting-out effects.The calcium ion binds to a certain protein to form a 1:1 complex. The following data was obtained in an experiment: Total Ca2+/uM 60 120 180 240 480 Ca2+ bound to Protein/uM 31.2 51.2 63.4 70.8 83.4 Determine graphically the dissociation constant of the Ca2+ - protein complex. The protein concentration was kept at 96uM for each run. (1uM = 1 x 10-6 M.)A chemical solute has a partition coefficient of 11.2 between hexane and water. If the equilibrium concentration of solute in hexane is 0.00815 M, the equilibrium concentration of the solute in water is: a)7.59 x 10-4 M b) 0.0913 M c)0.00815 M d)1.37 x 103 e)None of These
- In an experiment to determine the concentration of H2SO4 in a brand of toilet cleanser, 10.0 cm3 of the cleanser was first diluted to 250.0 cm3 with distilled water. 25.0 cm3 of the diluted cleanser were titrated with 0.320 mol dm–3 NaOH solution, using methyl orange as indicator.If 29.2 cm3 of NaOH solution is used, what is the concentration of H2SO4 in the undiluted toilet cleanser?a. 2.30 mol dm–3b. 4.60 mol dm–3 c. 9.20 mol dm–3 d. 11.50 mol dm–37 How is the variation in sample composition in a powder random mix influenced by the proportion of active ingredient particles and by the total number of particles in the sample?Construct the expression for Ksp for solid Ba3(P04)2 in aqueous solution. Вaз(РОa)2(s) — З Ва" (аq) + 2 РО,3 (aq) 1 Based on your knowledge of how the solid will dissociate in aqueous solution, use the tiles to form the expression. Ksp CRESET [Ba3(PO4)2] 2[Ba3(PO4)2 3[Ba3(PO4)2 [Ba3(PO4)2l [Ba3(PO4)21° [Ba*] 3[Ba*] [Ba*j [Ba"] 3[Ba2*] [PO,?] 2[PO,] [PO,7? [PO,] 2[PO,] [PO4 3[PO,°] [PO,]°
- You carry out an experiment on the physical properties of 3 organic compounds and observe the following data: Substance Solubility in Water medium Compound A low Compound B high Compound C You know that the 3 organic compounds are an alkene, ketone and alcohol of equal hydrocarbon length. Match the compounds above to their specific organic compound type based upon their solubility. And give a structural reason for the solubility observations that are observed. Substance Identity Reason Compound A Compound B Compound CGRAVIMETRIC ANALYSIS The addition of dimethylglyoxime, H2C4H602N2, to a solution containing nickel(Il) ion gives rise to a precipitate: Nickel dimethylglyoxime is a bulky precipitate that is inconvenient to manipulate in amounts greater than 135 mg. The amount of nickel in a type of permanent-magnet alloy ranges between 22% and 38%. Calculate the sample size that should not be exceeded when analyzing these alloys for a nickel.Two different proteins X and Y are dissolved in aqueous solution at 37 ∘C. The proteins bind in a 1:1 ratio to form XY. A solution that is initially 1.00 mM in each protein is allowed to reach equilibrium. At equilibrium, 0.17 mM of free X and 0.17 mM of free Y remain. What is Kc for the reaction? Express your answer using two significant figures.
- One technique for studying the energetics of protein structure is to monitor the struc- ture of the protein as a function of the osmotic pressure. In these experiments the os- motic pressure of the solution is varied by using polymers of different molecular weight and concentration. In an experiment poly(ethylene glycol) (PEG) with a molecular weight of 1000.0 g mol- was used. What concentration of PEG is needed to create an aqueous solution with an osmotic pressure of 150.0 Pa at 25°C? You may assume that PEG is uncharged and that it does not dissociate or aggregate.Liquid-liquid extraction is a means of separating the components of a mixture which relies on the differential partitioning of the components in two immiscible liquids. Define the term immiscible. O 1) Two solvents that do not mix together O 2) Two solvents that do mix togetherThe U.S. Federal Water Pollution Control Act P.L. 92-500,specifies legally acceptable methods for testing wastewater. The analysis of cyanide is performed according to the methods set out in the "Standard Methods for the Examination of Water Wastewater". This method indicates that it should be added prior to wastewater mercury chloride I (HgCl), to decompose the complex cyanides and thus be able to take a sample to perform the content analysis of cyanide. However, it should also be noted that the Illinois Pollution Control Board (IPCB) has established wastewater quality standards that limit the content of mercury (such as Hg) at 0.0005 ppm maximum in any effluent. A company that has a wastewater treatment plant applies 200 mg of mercury chloride per day before each cyanide analysis. a) Will this company comply with the maximum allowable limit of mercury according to the IPCB if discharges 100,000 gal/day of effluent and performs a daily cyanide analysis?