The calcium ion binds to a certain protein to form a 1:1 complex. The following data was obtained in an experiment: Total Ca2+/uM 60 120 180 240 480 Ca2+ bound to Protein/uM 31.2 51.2 63.4 70.8 83.4 Determine graphically the dissociation constant of the Ca2+ - protein complex. The protein concentration was kept at 96uM for each run. (1uM = 1 x 10-6 M.)
The calcium ion binds to a certain protein to form a 1:1 complex. The following data was obtained in an experiment: Total Ca2+/uM 60 120 180 240 480 Ca2+ bound to Protein/uM 31.2 51.2 63.4 70.8 83.4 Determine graphically the dissociation constant of the Ca2+ - protein complex. The protein concentration was kept at 96uM for each run. (1uM = 1 x 10-6 M.)
Principles of Modern Chemistry
8th Edition
ISBN:9781305079113
Author:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler
Publisher:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler
Chapter20: Molecular Spectroscopy And Photochemistry
Section: Chapter Questions
Problem 67CP
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The calcium ion binds to a certain protein to form a 1:1 complex. The following data was obtained in an experiment:
Total Ca2+/uM | 60 | 120 | 180 | 240 | 480 |
Ca2+ bound to Protein/uM | 31.2 | 51.2 | 63.4 | 70.8 | 83.4 |
Determine graphically the dissociation constant of the Ca2+ - protein complex. The protein concentration was kept at 96uM for each run. (1uM = 1 x 10-6 M.)
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