Q10.1: Answer the following three-part question. a) Calculate the ΔEº’ for the citrate cycle reaction converting malate to oxaloacetate and describe how you calculated this value. b) What is the overall change in standard free energy (ΔGº') for this reaction and describe how you calculated it. c) Do you think the actual change in free energy (ΔG) for this reaction differs significantly from the standard free energy (ΔGº') for the reaction, and if so, why is it different?

Q10.1: Answer the following three-part question. a) Calculate the ΔEº’ for the citrate cycle reaction converting malate to oxaloacetate and describe how you calculated this value. b) What is the overall change in standard free energy (ΔGº') for this reaction and describe how you calculated it. c) Do you think the actual change in free energy (ΔG) for this reaction differs significantly from the standard free energy (ΔGº') for the reaction, and if so, why is it different?

Biochemistry

9th Edition

ISBN:9781305961135

Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Publisher:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Chapter6: The Behavior Of Proteins: Enzymes

Section: Chapter Questions

Problem 27RE: MATHEMATICAL The kinetic data in the following table were obtained for the reaction of carbon...

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Calculate ΔG°′ for the reaction A + B ⇌ C + D at 25°C when the equilibrium concentrations are [A] = 10 μM, [B] = 15 μM, [C] = 3 μM, and [D] = 5 μM. Is the reaction exergonic or endergonic under standard conditions?
a) Calculate the enzyme and specific activity of a reaction with 3 μM Hsp90 using the following information: The rate is measured in a spectrophotometer as 0.028 OD units/min in a 1 ml reaction volume. The absorbance was detected at 340nm and the extinction coefficient for NADH at this wavelength is 6200 L M-1 min-1 and the molecular mass of Hsp90 is 82.7 kDa. The rate of NADH utilisation is equivalent to the rate of ATP utilised by Hsp90. Show all your calculations and the units for your answers. b) Calculate the turnover number for the reaction described in (a) above
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MATHEMATICAL The enzyme -methylaspartase catalyzes the deamination of -methylaspartate [V. Williams and J. Selbin, J. Biol. Chem. 239, 1636 (1964)]. The rate of the reaction was determined by monitoring the absorbance of the product at 240 nm(A240). From the data in the following table, determine KM for the reaction. How does the method of calculation differ from that in Questions 26 and 27?
MATHEMATICAL The kinetic data in the following table were obtained for the reaction of carbon dioxide and water to produce bicarbonate and hydrogen ion catalyzed by carbonic anhydrase: CO2+H2OHCO3+H+ [H. De Voe and G. B. Kistiakowsky, J. Am. Chem. Soc. 83, 274 (1961)]. From these data, determine KM and Vmax for the reaction. CarbonDioxideconcentration(mmolL1)1/Velocity(M1sec)1.252.55.020.00.25036103201031210361030.256
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