OMe OMe OH HO HO- HO OMe H+ ong HO- HO OMe Pre-practical "MUST DO" to be included in your lab-book: 1. Calculate MW of all reagents, moles and theoretical yield 2. Reaction mechanism 3. Which is the limiting reagent? Why? 4. What is the role of trietylamine? 5. You are treating the TLC plate with 5% of sulfuric acid in ethanol. Look this up and briefly describe how it works
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- (Please answer all questions) 5-33. What are some advantages of using column chromatography to purify reaction products in the microscalelaboratory? 5-34. Discuss the similarities and dissimilarities of TLC, paper, and column chromatography. 5-35. Discuss the similarities and dissimilarities of HPLC and gas chromatography. 5-36. (a) What are the main advantages of using flash chromatography?(b) How can TLC be used in connection with flash chromatography? 5-37. Using the information presented on the right, please identify and explainwhich spot has an Rf value of 0.5.Chrome → C esc File Edit View History Aktiv Chemistry OH ! 1 app.101edu.co Q oxidation 2 W Bookmarks Profiles Tab Window Help X + 1,503 # Below is a TLC of the oxidation of cyclohexanol to cyclohexanone. Based on the TLC plate, which of the following statements would be true about the progress of the reaction? (SM starting material; R = reaction mixture) 3 -t-t-t-t SM R 11 $ 4 JUN 30 % 5 ERT 121 6 MacBook Pro & PACES 7 W Y U O A) All of the starting material has been converted to product. 8 B) None of the starting material has been converted to product. 1 8 15% 9 C) The reaction solution is a mixture of starting material and product. D) The TLC does not provide any information on the reaction. 2 0 Thu 8:08 PM 0 K Paused P { : ( Up } ]ve After performing a reaction a TLC plate was run to identify the product. Below is the completed TLC plate. In lane A are the starting materials. In lane B is the sample taken after the reaction was complete. Did this reaction go to completion? Defend your answer with the TLC results. Edit View Insert Format Tools Table 12pt Paragraph BIUA V > 2 T²:
- Styles Voice Text in Portuguese is not being checked. Do you (i) MISSING PROOFING TOOLS want to download proofing tools and future Sensitivity Editon Download updates? 一 4 7. 10 1 11 | 12 17. What is the main side effect of anti-epileptic drugs? a. Hypertension b. Drowsiness c. Tachycardia I. d. Insomnia e. Euphoria I believe these drugs are like the ones of the work- dihidropyridines-cont 18. What is metformin used for as first choice of treatment, and why? a. Type II diabetes, because it regulates blood glucose levels b. Hyperthyroidism, because it inhibits the formation of T3 an idirm hecause it doesn't interact with other drug 9.1 7. 8.Table 2. Calculations ~60°C trial ~50°C trial ~40°C trial ~30°C trial ~20°C trial Vol. of HCI used (mL) 19.87 12.93 12.37 9.03 6.30 Moles of HCI used (mol) 9.94 6.47 6.19 4.52 3.15 Moles of borate present (mol) [Borate] (M) Ksp In(Ksp) 1/T (K-1)Table 2. Optimum temperature determination for novel a-amylase Volume of 0.1% Volume of pH 6.7 buffer (mL) Test Volume of 0.9% Pre-incubation Absorbance Tube w/v starch (mL) NacI (mL) (°C) 1 3.50 1.00 0.50 30 0.276 2 3.50 1.00 0.50 40 0.320 3 3.50 1.00 0.50 50 0.422 4 3.50 1.00 0.50 60 0.288 3.50 1.00 0.50 70 0.124 6. 3.50 1.00 0.50 80 0.195 B. One drop (negligible volume) of a relatively concentrated enzyme solution was added in each test tube. After ten minutes, the reactions were terminated and one drop of DNS reagent (negligible volume) was added. Absorbance were measured at the same wavelength above. What is the optimum temperature for the amylase enzyme? Justify your answer.|
- 3) 3-Methylbutyramide 100 relative intensity .8888 relative abundance .88 CHINO 101 298 888 mass charge (m2) CaH₁N 60 4) n-Butylamine 100 60 CHOCHOCHS R98 8 mass charge (m/z) MW=101.15 MW-73.13Based on the data, calculate the concentration of analyte in the ORIGINAL sample in ppm. Report to 1 decimal place Sample: A 10.00 mL aliquot of the unknown was placed in a 100.0 mL volumetric flask and made up to the mark will distilled water. The absorbance of this solution is 0.185. Sample + Spike: A 10.00 mL aliquot of the unknown and a 13.00 mL aliquot of the 46.00 ppm stock were both placed in the same 100.0 mL volumetric flask and diluted to the mark with water. The absorbance of this solution is 0.326. ( Please type answer note write by hend)2. The fluorenone reduction reaction ran for 3 minutes. A TLC was done to monitor the progression of the reaction. According to the TLC, the product started to form at 30 seconds and completed forming at 2 minutes. Sketch a TLC plate monitoring the progression of this reaction for the duration of 3 minutes (interval at 0 sec., 15 sec., 30sec., 60 sec., 120 sec., 180 sec.).
- A reaction mixture spotted on the TLC plate has been refluxing for one hour. There is a single organic product of the reaction. After 1 hour a student elutes a TLC plate of the starting material(s) (SM), the known product (P) and the reaction mixture (RM). Based on the TLC below, do you think the reaction should be refluxed further? Explain your answer. SM+ P + RM+ O O O1. Define sparging. What is the significance of this step in an HPLC analysis? 2. What are the two types of pre-column? What is the purpose of each type? 3. What are the two types of packing used in HPLC column? Describe each type. Please answer numbers 1-3. Need asapHow you can improve resolution in HPLC analysis