Table 2. Optimum temperature determination for novel a-amylase Volume of pH 6.7 buffer (mL) Test Volume of 0.1% Volume of 0.9% Pre-incubation Absorbance Tube w/v starch (mL) NacI (mL) (°C) 1 3.50 1.00 0.50 30 0.276 3.50 1.00 0.50 40 0.320 3 3.50 1.00 0.50 50 0.422 4 3.50 1.00 0.50 60 0.288 3.50 1.00 0.50 70 0.124 3.50 1.00 0.50 80 0.195 B. One drop (negligible volume) of a relatively concentrated enzyme solution was added in each test tube. After ten minutes, the reactions were terminated and one drop of DNS reagent (negligible volume) was added. Absorbance were measured at the same wavelength above. What is the optimum temperature for the amylase enzyme? Justify your answer. LO

Table 2. Optimum temperature determination for novel a-amylase Volume of pH 6.7 buffer (mL) Test Volume of 0.1% Volume of 0.9% Pre-incubation Absorbance Tube w/v starch (mL) NacI (mL) (°C) 1 3.50 1.00 0.50 30 0.276 3.50 1.00 0.50 40 0.320 3 3.50 1.00 0.50 50 0.422 4 3.50 1.00 0.50 60 0.288 3.50 1.00 0.50 70 0.124 3.50 1.00 0.50 80 0.195 B. One drop (negligible volume) of a relatively concentrated enzyme solution was added in each test tube. After ten minutes, the reactions were terminated and one drop of DNS reagent (negligible volume) was added. Absorbance were measured at the same wavelength above. What is the optimum temperature for the amylase enzyme? Justify your answer. LO

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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