Imagine you are attempting to separate these three dipeptides from each other using anion exchange chromatography, and you use a protein assay to identify at what salt concentration each of them is eluted from the column. A graph showing protein concentration as a function of salt concentration is shown below the dipeptides. Which of the three dipeptides is represented by peak #3? (NOTE: the dipeptides are shown in their non-ionized forms. You must assume that they are in aqueous solution at physiological pH, ~7.2) a) Dipeptide A  b) Dipeptide B c) Dipeptide C

Pushing Electrons
4th Edition
ISBN:9781133951889
Author:Weeks, Daniel P.
Publisher:Weeks, Daniel P.
Chapter3: Mechanisms
Section: Chapter Questions
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Imagine you are attempting to separate these three dipeptides from each other using anion exchange chromatography, and you use a protein assay to identify at what salt concentration each of them is eluted from the column. A graph showing protein concentration as a function of salt concentration is shown below the dipeptides. Which of the three dipeptides is represented by peak #3? (NOTE: the dipeptides are shown in their non-ionized forms. You must assume that they are in aqueous solution at physiological pH, ~7.2)

a) Dipeptide A 

b) Dipeptide B

c) Dipeptide C

 

3
0 10 20 30 40 50
[NACI] (mM)
A260
2.
Transcribed Image Text:3 0 10 20 30 40 50 [NACI] (mM) A260 2.
В.
С.
СООН
CH,OH
|
CH2
А.
СООН
H.
H
CH2
CH2
O:
H
|
H
C
HO
|
NH2
НО
C
NH,
НО
|
CH2
NH2
H
||
CH2
||
CH2
СООН
CH2
СООН
HN
Transcribed Image Text:В. С. СООН CH,OH | CH2 А. СООН H. H CH2 CH2 O: H | H C HO | NH2 НО C NH, НО | CH2 NH2 H || CH2 || CH2 СООН CH2 СООН HN
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