An ion-exchange chromatographic separation is performed using a diethyl-aminoethyl-sepharose (DEAE-sepharose) column to separate proteins in a mixture. These proteins include Protein A (pl=6.0), B (pl=5.0), C (pl=7.5), D (pl = 1), and E (pl=4.0). After the protein mixture is prepared in a buffer solution pH = 5 and loaded onto the column, and the column is washed with a buffer solution pH 5. Which protein(s) is(are) eluted out the column with the wash solution? Proteins B andE because both predominantly have net positive charges Proteins D and E because they predominately have net negative charges O Only D because its pl is very low O Protein C because at pH lower than its pl Protein C molecules have net positive chage slighlty more O Proteins A and C because both both predominantly have net positive charges

An ion-exchange chromatographic separation is performed using a diethyl-aminoethyl-sepharose (DEAE-sepharose) column to separate proteins in a mixture. These proteins include Protein A (pl=6.0), B (pl=5.0), C (pl=7.5), D (pl = 1), and E (pl=4.0). After the protein mixture is prepared in a buffer solution pH = 5 and loaded onto the column, and the column is washed with a buffer solution pH 5. Which protein(s) is(are) eluted out the column with the wash solution? Proteins B andE because both predominantly have net positive charges Proteins D and E because they predominately have net negative charges O Only D because its pl is very low O Protein C because at pH lower than its pl Protein C molecules have net positive chage slighlty more O Proteins A and C because both both predominantly have net positive charges

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter34: Miscellaneous Separation Methods

Section: Chapter Questions

Problem 34.1QAP

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