Frog muscle cells and the solution bathing the cells contain ions at different concentrations. The concentrations of Na+, Ca²+, K+ and Cl¯ are shown in the table. The temperature is 25 °C. ion intracellular concentration (MM) extracellular concentration Answer: -0.0704 Na+ 12 120 Ca²+ 1 x 10-4 3 V K+ ◆ 155 10 CI- (MM) The resting frog muscle membrane potential is determined by the equilibrium of K+ across the cell membrane. What is the voltage inside the frog cell if the outside solution is at zero volts? 4 62
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- Describe the contribution of each of the following to establishing and maintaining membrane potential: (a) the Na+K+ pump, (b) passive movement of K+ across the membrane, (c) passive movement of Na+ across the membrane, and (d) the large intracellular anions.Calculate the equilibrium membrane potentials to be expected across a membrane at 37 ∘C, with a NaCl concentration of 0.50M on the "right side" and 0.08 M on the "left side", given the following conditions. In each case, state which side is (+) and which is (−). Membrane permeable only to Cl−.Calculate the equilibrium membrane potentials to be expected across a membrane at 37 °C, with a NaCl concentration of 0.10 M on the “right side” and 0.01 M on the “left side”, given the following conditions. In each case, state which side is (+) and which is (-). (a) Membrane permeable only to Na+ (b) Membrane permeable only to Cl– (c) Membrane equally permeable to both ions
- You have a semi permeable membrane with a membrane potential of -90mV. You also have two ions that are both permeable to the membrane, Na and Cl. Na has a concentration of 10mM inside the membrane and 120mM outside the membrane. Cl has a concentration of 1.5mM inside the membrane and 77.5mM outside the membrane. Use the nernst equation to calculate the electrochemical equilibrium of both ions, and show in which direction the netflux would be for each ion.For a typical vertebrate cell with a membrane potential of −0.070 V (inside negative), what is the free-energy change for transporting 1 mol of Na+ from the cell into the blood at 37 °C? Assume the concentration of Na+ insidethe cell is 12 mM and in blood plasma it is 145 mM.Chloride ions (Cl-) behave a bit differently to Na+ and K+ in that most cells don't have active Cl- transporters. As a result, the concentration gradient for Cl- is not 'set' like it is for Na+ and K+. There are, however, a limited number of Cl- leak channels in the cell membrane. As a result, Eci generally matches resting membrane potential - around - 70mV. Considering this, answer the following questions. If Cl- can cross the cell membrane, is not being actively transported, and membrane potential is -70mV, will there be a concentration gradient for Cl-?
- Below find the structures for ibogaine and cocaine. Ibogaine and cocaine inhibit the dopamine active transporter (DAT). This transporter is a secondary active transporter, and depends on the primary active transporter Na+/K+ ATPase. Ibogaine had a Kι = 2 μM, and cocaine a Kι = 0.64 μM respectively. (a) Define secondary active transport. (b) Is ibogaine an effective treatment for cocaine based on DAT binding?For most neurons, the extracellular concentration of chloride ions (Cl-) is 108 mM, whilethe intracellular concentration of Cl- is 5 mM.If the plasma membrane becomes more permeable to Cl-, would there be Clinflux or Cl- efflux at an RMP of -70 mV? Why?V=62 log 10 (C0/Ci ) for a positive ion at 37 degrees Celsius. What is theoretical ratio of solution ion across the membrane when the resting membrane potential is 124 mV?
- It is typically sufficient to rupture cells when the solute concentration is reduced from 0.15M to 0.001M. Calculate what transmembrane pressure this would result in. Use that to access if the red blood cells would break. Yes or No? Compare to the transmembrane pressure when cells are in normal saline solution (0.91%NaCl) -> 0.156M(change unit to osM) Basically Calculate the transmembrane pressure when the solute concentration is reduced from 0.15M to 0.001M Determine if that transmembrane pressure would result in the breakage of red blood cells Calculate the transmembrane pressure when cells are in a normal saline solution and compareMany cells in the human body maintain an electric potential difference across their cellular membranes, typically through the use ion-specific pumps and channels that generate an excess of negative charges on the inside of the cellular membrane and an excess of positive charges on the outside. Let us estimate the total energy stored in the human body by this type of charge separation.if an object b has a plasma sodium concentration of 135mOsm/L and an intracellular concentration of 4mOsm/L. It also has a plasma concentration of potassium of 20mOsm/L and an intracellular concentration of 200mOsm/L. studies identify that the cells have a permeability to potassium that is 10 times greater than sodium. What is the resting membrane potential