A mixture of two proteins (both with molecular mass of 50 kDa) with an isoelectric points of 7.8 and 8.9, respectively, is in a buffered solution at pH 7. Which type of liquid chromatography would be used to separate these two
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- In gravimetric analysis, a colloidal product is obtained when Select one: a. The nucleation process is major b. The relative super saturation (RSS) is small Oc. Particles size exceeds 0.02 mm d. The concentration of solute at anytime is very small e. Crystal growth is predominantIndicate the contrast statements of the following chromatographic techniques of separation. A. A separation of components mixture based on migration rates in a stationary phase by a gaseous or liquid mobile phase. B. The supported materials coated of flat plate or in fibres of paper stationary phase is known as planar chromatography. C. The rate of migration of molecules or analytes is depending on the relative solubility of the solute in the stationary phase and mobile phase. D. Involve an electronic transition of valence shell electron in free atoms to provide analytical information about sample composition.You have an aqueous solution of hemoglobin (pl = 6.8) at pH 6.5, and currently the proteins are soluble. If you had 1 M HCl and 1M NaOH at your disposal, what could you do to precipitate the albumin out of solution? O add NaOH to the solution add HCl to the solution add NaOH to the solution, then add equal volume of HCl to the solution O add HCl to the solution, then add equal volume of NaOH to the solution An error has been detected in your answer. Check for typos, miscalculations etc. before submitting your answer. Submit Answer Try Another Version 1 item attempt remaining
- Given the equation of the line, y=150x-8.1221 (cells per mL is in 10^8), what is the CFU per mL of the original suspension if the thousand-fold dilution of that suspension has an absorbance of 0.3 at 600nm? * A. 3.7 x 10^12 B. 3.7 x 10^9 C. 3.7 x 10^1 D. None of these is correctConsider a mixture of two proteins with molecular weights of 20,000 and 200,000. For simplicity of calculation, both may be approximated as unhydrated spheres with VB 0.740 mL g1. a. Calculate the ratio of the sedimentation coefficients of the proteins in the same medium (density=1.05 g mL¯1) at the same temperature.A solution contains 1.0 g of ephedrine sulfate in a volume of 100 mL. What quantity of sodium chloride must be added to make the solution isotonic? E value for the drug is 0.23.
- A mixture containing glutamic acid, arginine, phenylalanine and valine was subjected to anion exchange chromatography at pH 7.0. Which of the amino acids interacted with the resin (anion exchanger) and last to be eluted with a buffer of decreasing pH? A. Glu B. Val C. Phe D. ArgGuanosine (C10H13N5O5) in solution has a maximum absorbance at a wavelength of 275 nm. The molar extinction co-efficient at this wavelength is 84M−1cm−1and the path length is 24.7 cm. Through the use of a spectrophotometer, it is found that the that A275= 1.48. What is the concentration of the guanosine solution in grams/litre? Molecular weights (g/mol): C-12, H-1, N-14, O-16 Select one: A. 0.201869 g/L B. 0.435188 g/L C. 0.059919 g/L D. 0.294046 g/L E. 0.000713 g/LYou had a second solution with an unknown concentration of Protein X that had to be diluted 4x before you could measure the absorbance. The diluted solution had an absorbance of 0.76. What is the concentration of the diluted solution in ug/ml and what is the concentration of the original solution
- at 257 nm) is dissolved in 2.5 mL of buffer. A 250 microliter aliquot is removed and placed in a 1.0 cm A sample of adenosine triphosphate (ATP) (MW 507, ɛ = 14,700 M'cm cuvette with sufficient buffer to give a total volume of 2.0 mL. The absorbance of the sample at 257 nm is 2.0. Calculate the weight of ATP in the original 2.5 mL sample. (Choose the correct value from the drop-down list provided. The values of weight are listed in units of milligrams.) Weight of ATP: v mgConsider a buffer solution of acetate. The volume is 500 ml, the concentration is 200 mM, and the pH is 5.0. a. How many total moles of acetate plus acetic acid are present in the solution? Express answer as x.y with one digit before and one after the decimal place. b. What is the ratio of acetate ions (Ac-) to acetic acid ions (HAc) in the buffer solution (pH 5.0) if the pka is 4.76? Express the answer as x.yz with one digit before and two after the decimal place. c. How many moles of acetate are present in the solution? Express your answer to one decimal place. d. How many moles of acetic acid are present in the solution? Express your answer to one decimal place.20 mL solution is 2 M NaOH. If 0.5 M HCl is to be added to this solution, calculate the pH of the resulting solution: A. when 10 mL of the 0.5 M HCl is added to the original solution of the base (initial pH). B. When 50 mL H20 is added to the original solution of the base.