Use the dilution scheme to answer. The concentration of bacteria in the original sample is CFU/ml. 0.1 ml 0.1 ml 0.5 ml 0.1 ml A 43 CFUS 9.9 ml 9.9 ml 9.5 mL original- sample )A. 8.60 x 102 OB. 8.60 x 107 OC. 430 x 107 D. 4.30 x 108 OE. 8.60 x 106
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- Clinical case: Mrs. P, patient with neurological signs suggesting meningitis Your 45 year old patient (who has recently moved from Great Britain where sey were born and raised) presents with severe headaches, stiffness in the neck, confusion and drowsiness. . You suspect meningitis, a life threatening infection of the tissue surrounding the brain and spinal cord. • You take a cerebrospinal fluid (CSF) sample from the patient and send it to the micro lab for examination and culturing. • Later that day the micro lab reports that no bacteria nor fungi were observed on a direct smear of the CSF. In 3 days, the lab reports that no bacteria nor fungi grew in cultures. • The lab also ran PCR for the most common viruses causing meningitis and all were negative. ELISA's checking for helminth and protozoal infections were also negative. Blood work suggests there is no immune response to the pathogen causing the patients clinical signs and symptoms.Please help me answer correctly the table this is my assignment in biochem lab Procedure: 1. Prepare 5 test tubes. Place 1 ml of 1% starch and add 10 drops of saliva to each tube. Mix thoroughly. 2. Place the first tube in ice water, the 2nd tube leave at room temperature, the 3rd tube in 40°C , the 4th tube at 60°Cwater bath and the 5th tube boil for 2 minutes.. 3. Leave the 4 tubes in their respective temperatures for 30 minutes. The 4th tube allows to stand for 30 minutes after heating for 2 minutes. 4. Test the contents of each tube with iodine and benedict’s tests.BONUS (15 points) The fallowing series of dilution was prepared from a specimen to determine the number of bacteria. There were 62 colonies on the agar plate prepared by transferring 0.3 ml from the tube number 4. Calculate the dilution factors for each tube. What is the cell concentration in the original specimen? Calculate the total number of cells in tube number 2. étv A) F12 F9 F10 F7 % & %3D delete 5 { T Y J K
- Question:- Your medical care team determines that a third patient's severe illness is caused bya helminth. Which technique or techniques described above would you use to identifythe helminth? Justify your answer.Question 14 Which of the following tests requires a dilution of the culture before inoculation? O Bacitracin Bile esculin agar O DNase O CoagulaseHelping tags: Biology, bacterial count, dilution, serial dilution WILL UPVOTE, just pls help me answer the following questions and explain them clearly. Pls show complete solutions also for the computation part. Thank you. 1. A bacterial culture was grown for 9 hours. At 3-hour interval, the culture was sampled to determine the population of the culture, by transferring 25 ml of the suspension to 225 ml 0.85% NaCl. Three consecutive dilutions were further made by using 1 ml aliquot in 9 ml of 0.85% NaCI. One ml from each dilution was plated in each of duplicate plates. The following table shows the results of the plating method. Sampling COUNTS 2nd dilution 3rd dilution 4th dilution 0,0 30: 35 250;245 1st dilution 1st 2nd 3rd 0; 0 240; 235 TNTC 55; 60 5; 6 TNTC TNCT TNTC TNTC a) Illustrate the dilution series used and label the final dilution of each dilution. b) Determine the bacterial count (CFU/ml) every 3 hours of incubation for 9 hours. Show all computations.
- this lab was carried out the results are in the picture below plz provide a aim for this lab abd answer thw questions below the method used is also stated Title: Bacterial Inhibition Aim: Materials: Streak plate from LAB # 3 Disks Ethanol (dilutions of 20%, 50%, 70%, 95%) Burner Flame Matches Tape Method: Ethanol was used to clean the work station A maker was used to dived each equal sections and ruler and was label with appropriate concentrations. The plate carefully open The disc was soak in the different concentrations of ethanol Placed one disc per concentration for each labelled section The plate was tape shut The plate was returned to the incubator The zone of inhibition was recorded after 3 days. question 1 state a aim question 2 in a table format plz explain the bacteria growth of e.coil and staph and state any two limation of this labQuestion 15 Which test require a straight line inoculation? Choose all that apply. DNA hydrolysis Bile esculinase O NaCl O HemolysinPlease help me answer the observed color of the sample 5. Bial’s-Orcinol test – place 5 drops each of the sugar solution (fructose, glucose and xylose) into a clean separate test tube. Add 1 ml of bial’s-orcinol reagent into each tube. Place the tubes in a boiling water bath. Note the color change during the 5 minutes of heating.
- ANSWERS TO QUESTIONS ON LABORATORY ASSAY NO.2 1. Why is it necessary to calibrate a Pasteur pipette? 2. Cite some possible sources of errors in the calibration of Pasteur Pipette. 3. Cite other procedural techniques that can be used for the calibration of Pasteur Pipette.Answer each of the questions below using the plate images provided. Nutrient Agar 1 EMB Blood Agar 1. How many colonies are on NA plate 3?. 2. How many bacteria were transferred to NA plate 3?. 3. How many colonies are on NA plate 2? 4. How many bacteria were transferred to NA plate 2?. 5. How would you describe the growth on NA plate 1?. 6. How many colonies grew together on NA plate 1? 7. How many bacteria were transferred to NA plate 1? Nutrient Agar 2 8. How many bacteria collected from the source? (Hint: What fraction of tube 1 was spread on NA plate 1?) 9. Were any of the bacteria from the source Gram-negative? How can you tell?. 10. Could any of the bacteria from the source ferment lactose? How can you tell? Nutrient Agar 3 11. Were any of the bacteria from the source hemolytic? How can you tell?. 0019% O Mon 10:07 PM Share Window Help A Final Review Edited 125% v View Zoom Add Page Insert Table Chart Text Shape Media Comment 39. Ticarcillin 700 mg is ordered. Ticarcillin is supplied as a powder in a vial. Directions on the vial state “add 2 mL of sterile water for injection. Each 2.6 mL of solution will contain 1 gram of ticarcillin. How many mL should be adminis ered per dose?
