indicators, key used for/ determines reagents, or key positive result ingredients |1. crystal violet Comments/ Medium/test negative result additional info decolorization is key. Must include +/- controls on gram reaction and size and 2. iodine morfdant Gram stain stains purple stains pink 3. gram's declorizer 4. safranin shape every slide to verify result quadrant streak- isolation of pure culture of bacteria check this for any TSA pigmentation of colonies Eosin methylene blue agar (EMB) 1. no blackening of medium 2.same color of added multi test medium (3 results reagent (not red) 3. stab is visible an defined with growth 1. blackening of medium 2. red color in added 1. sulfur/H2S 2. indole/tryptophasnase 3. motility/flagella 1. ferrous salts reagent 3. fuzzy appearance of medium/stab not visible SIM 2. Kovac's in one medium) 3. low % agarose motility agar Brewer's plate in Anaerobe jar Fluid thioglycollate (FTM) Citrate slant phenol red fermentation broth test media (list the
indicators, key used for/ determines reagents, or key positive result ingredients |1. crystal violet Comments/ Medium/test negative result additional info decolorization is key. Must include +/- controls on gram reaction and size and 2. iodine morfdant Gram stain stains purple stains pink 3. gram's declorizer 4. safranin shape every slide to verify result quadrant streak- isolation of pure culture of bacteria check this for any TSA pigmentation of colonies Eosin methylene blue agar (EMB) 1. no blackening of medium 2.same color of added multi test medium (3 results reagent (not red) 3. stab is visible an defined with growth 1. blackening of medium 2. red color in added 1. sulfur/H2S 2. indole/tryptophasnase 3. motility/flagella 1. ferrous salts reagent 3. fuzzy appearance of medium/stab not visible SIM 2. Kovac's in one medium) 3. low % agarose motility agar Brewer's plate in Anaerobe jar Fluid thioglycollate (FTM) Citrate slant phenol red fermentation broth test media (list the
Chapter15: Antibiotic Agents
Section: Chapter Questions
Problem 20RQ
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