calculate the [PNP] in μM for each of these samples. For Table 2: Calculate the volumes of the 100 mM PNPP stock solution and buffer (final volume of 1.00 ml) needed to get each of the substrate concentrations.
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For Table 1:
calculate the [PNP] in μM for each of these samples.
For Table 2:
Calculate the volumes of the 100 mM PNPP stock solution and buffer (final
volume of 1.00 ml) needed to get each of the substrate concentrations.
![(WATCH YOUR UNITS OF MEASUREMENT)
Buffer
(μl)
[PNP] in uM
1500
1490
1480
1470
1460
5
1450
6
1440
7
75
1425
8
100
1400
Note that in the PNP column, uM = μmoles/liter.
Table 1
Tube #
Blank
1
2
3
+10
4
PNP (μl)
0.5 mM stock
0
10
20
30
40
50
60
Trial 1
A405
Trial 2
A405](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F51044012-1e7d-4f53-9311-c2b96c730020%2F5d6df19a-c6fe-4ff0-94ab-39efc93091f0%2Fckg6w7sr_processed.jpeg&w=3840&q=75)
![Table 2
Tube #
Blank
1
2
3
4
5
PNPP (ul)
100 mM stock
0
Buffer (μl)
1000
Enzyme
(μl)
0
5
5
5
5
5
Final volume
(μl)
1000
1000
1000
1000
1000
1000
[PNPP] in uM
0
200
400
600
800
1000](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F51044012-1e7d-4f53-9311-c2b96c730020%2F5d6df19a-c6fe-4ff0-94ab-39efc93091f0%2Fj9zsw2u_processed.jpeg&w=3840&q=75)
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