Below are morphological, physiological and biochemical characteristics of an unknown luminous bacterium isolated from the gills of a marine fish. Use the data below to identify the isolate. Make an identification scheme Test Unknown Isolate B 1 Luminescence Positive 2 Cell Shape Straight Rods 3 Gram Staining Negative 4 Oxygen Requirement Facultative Anaerobe 5 Motility (Hanging Drop) Motile 6 Growth at 0% NaCl Negative 7 3% NaCl Positive 8 6 % NaCl Positive 9 Accumulation of PHB Positive 10 Flagella 1 Polar 11 Oxidase Positive 12 Nitrate Reduction 13 Gelatinase Negative 14 Growth at 35OC Positive 15 Growth at 4OC Negative 16 Methyl Red Positive 17 Voges-Proskauer Positive 18 Catalase Positive 19 Starch Hydrolysis Negative 20 Lysine Decarboxylase Negative 21 Indole Production Negative 22 Utilization of 23 Glucose Positive (fermentative) 24 Lactose Positive (fermentative) 25 Mannitol Negative 26 Sucrose Negative 27 Xylose Positive (fermentative) 28 Inositol Negative 29 Galactose Positive (oxidative)
Microscopic examination
The analysis of minute organisms, cellular organization of any biological structure, and composition of body fluids with the help of a microscope is known as microscopic examination. The magnification of specimens or samples under study helps in attaining a clearer picture of it.
Gram Staining
Named after Hans Christian Gram, a Danish bacteriologist, Gram stain is one of the most powerful staining techniques within microbiology. This technique was introduced in 1882 to identify pneumonia-causing organisms. The Gram staining technique uses crystal violet or methylene blue as primary staining colors to distinguish gram-positive from gram-negative organisms. Under a microscope, the gram-positive organisms appear purple-brown, retaining the primary color. Gram-negative organisms appear pink or red as they do not acquire the color of the primary stain.
Below are morphological, physiological and biochemical characteristics of an unknown luminous bacterium isolated from the gills of a marine fish. Use the data below to identify the isolate. Make an identification scheme
|
|
Test |
Unknown Isolate B |
|
1 |
Luminescence |
Positive |
|
2 |
Cell Shape |
Straight Rods |
|
3 |
|
Negative |
|
4 |
Oxygen Requirement |
Facultative Anaerobe |
|
5 |
Motility (Hanging Drop) |
Motile |
|
6 |
Growth at 0% NaCl |
Negative |
|
7 |
3% NaCl |
Positive |
|
8 |
6 % NaCl |
Positive |
|
9 |
Accumulation of PHB |
Positive |
|
10 |
Flagella |
1 Polar |
|
11 |
Oxidase |
Positive |
|
12 |
Nitrate Reduction |
|
|
13 |
Gelatinase |
Negative |
|
14 |
Growth at 35OC |
Positive |
|
15 |
Growth at 4OC |
Negative |
|
16 |
Methyl Red |
Positive |
|
17 |
Voges-Proskauer |
Positive |
|
18 |
Catalase |
Positive |
|
19 |
Starch Hydrolysis |
Negative |
|
20 |
Lysine Decarboxylase |
Negative |
|
21 |
Indole Production |
Negative |
|
22 |
Utilization of |
|
|
23 |
Glucose |
Positive (fermentative) |
|
24 |
Lactose |
Positive (fermentative) |
|
25 |
Mannitol |
Negative |
|
26 |
Sucrose |
Negative |
|
27 |
Xylose |
Positive (fermentative) |
|
28 |
Inositol |
Negative |
|
29 |
Galactose |
Positive (oxidative) |
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