CASE #9A patient with a human bite wound on the right forearm arrived at the clinic for treatment. Thewound was inflicted 36 hours earlier, and a culture was taken by the physician on duty. After 48hours, the culture results were:Gram stain: gram negative straight, slender rodsChocolate agar plate:Oxidase: positiveGlucose: positive"Pitting" of the agar by small, yellow, opaque coloniesMotility: negativeGrowth in increased CO2: positiveCatalase: negativeGrowth at 42°C: negativeWhat is the most likely identification of this facultative anaerobe?

Human bite wound infections are frequently polymicrobial, with a wide variety of aerobic and…

Answered: A patient with a human bite wound on…

Microbiology for Surgical Technologists (MindTap Course List)

2nd Edition

ISBN:9781111306663

Author:Margaret Rodriguez, Paul Price

Publisher:Margaret Rodriguez, Paul Price

Chapter4: The Prokaryotes

Section: Chapter Questions

Problem 5UTM

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A surgical technologist working in the Central Sterile Processing Department is asked to run the steam sterilizer (autoclave) with a biological monitor for the first load of the day without any instrument trays or items. What must be done with the bacterial sample after it is processed in the sterilizer?
What is the physical appearance (solid, liquid, semi-solid) of following media:Agar deep tube
A 1.5-year-old child developed vomiting, diarrhea, and fever. Stool sample were inoculated into the Endo media. After 18 hours on the surface of the medium grew medium-sized, round, slightly convex red colonies with a metallic luster. The doctor suspected Escherichia coli. 1. Name the composition of the Endo agar media. 2. Describe the properties of bacterial colonies on the Endo media. 3. What purpose differential diagnostic Endo media used for?
Determine what percentage of the culture was living (viable) and what percentage was dead (mortality). Plates Plate Dilution Volume plated No.of colonies Avg No Concentration of diluted sample Cd(cells/mL) Concentration of original sample Cu(cells/mL) 1 10^-3 10ul R1=130,R2= 110,R3=210 150 150mL 1.50*10^6 Volume of cells(mL) Volume of diluent(mL) Total dilution(D) Hemocytometer count Avg cells in 1 mm^2 area Concentration of diluted sample Cd(cells/mL) Concentration of original sample Cu(cells/mL) 4.3 0.5 0.1 grid 1= 171 , grid2 = 185 178 1.78*10^5 1.78*10^6
Topic: Preparation of Microbial Suspension A Pure colony is needed for inoculation in sterile water, a small portion of culture media was included. Effect: Rationale:
Match the description to the correct medium This medium is enrichment for fastidious [Choose ] bacterial pathogens and differential based on hemolysis [Choose ] This medium is selective based on Tryptic Soy Agar, TSA salt/sodium chloride tolerance at 7.5 % Sabouraud Dextrose Agar, "Sab" agar weight/volume and differential based on mannitol fermentation Mannitol Salt Agar, "MSA" MacConkey Agar, "Mac" agar This medium is selective based on the ability to grow in bile salts and crystal violet and is EMB Eosin Methylene Blue Agar differetial based on lactose fermentation Blood Agar, "BA" this medium is all purpose and can grow a [Choose ] wide range of bacteria and fungi
The students of a Microbiology class were tasked to transfer or subculture a pure culture of Escherichia coli bacterium in five 7 mL nutrient broth and five petri dishes of nutrient agar with 20 mL capacity each. Based on the instruction bottles for nutrient broth and nutrient agar, preparation of the culture media is as follows. Nutrient broth: 8 g/liter Nutrient agar: 28 g/liter Answer the following: a. What is the weight in grams of nutrient broth? b. What is the weight in grams of nutrient agar? c. What is the distilled water in mL for nutrient broth? d. What is the distilled water in mL for nutrient agar?
A class of 15 students (8 males and 7 females) will need to culture an unknown bacteria for a specific activity where 5 nutrient agar pates per female student and 3 agar slants per males student are needed to prepare. agarplate: 25mL Agar slant: 10mL Broth tube: 8mL Nutrient Agar: Yeast extract: 2g/L peptone: 5g/L Sodium chloride: 5g/L Agar powder: 15g/L
Below are photos of 3 bacteria, letter lableled, inoculated onto MSA plates and incubated. Note no growth on plate C MSA (Mannitol Salt Agar) Match the descriptions below to the correct letter labeled Bacteria This bacterium cannot grow in high salt
What will pose an ethical issue in these procedures are performed - sterilization?
What physical appearance (Solid, liquid, semi-solid) of following Media: Broth tube, agar slant tube, agar deep tube, and agar plate. What exactly am I testing when I Introduce an inoculum with a needle on an agar deep tube?
You were tasked to prepare four petri plates. Three of the petri dishes were inoculated using different samples. The plates were prepared from the following: Plate A: oral swab after brushing Plate B: cat’s paw Plate C: exposed in air for one hour Plate D: uninoculated The plates were incubated at 37˚C for 24 hours. The following results were obtained: 1. Compare the growth of the cultures found in Plates A, B, C and D. Which among the four plates has shown the most growth? Why do you think so? 2. Describe the appearance and abundance of the colonies found in your four plates. What possible type of microorganisms are found in your plates? 3. Although sparse, there is still visible growth of microorganisms in Plate D (uninoculated plate). Where do you think the microorganism came from? REFERENCES:

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