Microbiology with Diseases by Taxonomy (5th Edition)
5th Edition
ISBN: 9780134019192
Author: Robert W. Bauman Ph.D.
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Videos
Textbook Question
Chapter 6, Problem 23CT
Viable plate counts are used to estimate
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
You do a serial dilution on an actively growing bacterial culture broth. Doing a plate count of your final serial dilution gives you 30 CFU/mL. What is your original concentration of bacterial culture broth if you completed two 1:5 serial dilutions?
A microbiologist inoculates a growth medium with 100 bacterial cells/ml. If the
generation time of the species is 1 hour, and the microbes are in the exponential (log)
phase of growth, how many hours require for the culture to surpass 10,000 cells/ml,
if the culture is checked once each hour?
O 1) 2 hours
O 2) 7 hours
O 3) 3 hours
O 4) 10 hours
O 5) 24 hours
You are given a bacterial culture which has a concentration of approximately 5.0 x 10^8 cells/mL. List a series of dilutions and platings that you could carry out in order to determine the exact concentration of the culture. Note that you must plate four plates from a minimum of two dilution tubes. The volumes plated should be in the range of 0.1 mL – 1.0 mL. Duplicate volumes may not be plated from any one dilution tube. Each plating should aim for a count between 30 and 300 CFUs.
You can select any value from 30-300 for CFU and any volume from 0.1-1.0 to find out dilution scheme
Chapter 6 Solutions
Microbiology with Diseases by Taxonomy (5th Edition)
Ch. 6 - Prob. 1MCCh. 6 - In the laboratory, a sterile inoculating loop is...Ch. 6 - Superoxide dismutase _____________. a. causes...Ch. 6 - The most reactive of the four toxic forms of...Ch. 6 - Microaerophiles that grow best with a high...Ch. 6 - Prob. 6MCCh. 6 - Organisms that preferentially may thrive in icy...Ch. 6 - Prob. 8MCCh. 6 - Prob. 9MCCh. 6 - In a defined medium, ______________. a. the exact...
Ch. 6 - Prob. 11MCCh. 6 - Prob. 12MCCh. 6 - Prob. 13MCCh. 6 - Lyophilization can be described as ___________. a....Ch. 6 - Prob. 15MCCh. 6 - Prob. 1FIBCh. 6 - Prob. 2FIBCh. 6 - Prob. 3FIBCh. 6 - Prob. 4FIBCh. 6 - Prob. 5FIBCh. 6 - Prob. 6FIBCh. 6 - Prob. 7FIBCh. 6 - Prob. 8FIBCh. 6 - Prob. 9FIBCh. 6 - Prob. 10FIBCh. 6 - Prob. 11FIBCh. 6 - Prob. 1VICh. 6 - Prob. 2VICh. 6 - High temperature affects the shape of particular...Ch. 6 - Support or refute the following statement:...Ch. 6 - Prob. 3SACh. 6 - Why must media, vessels, and instruments be...Ch. 6 - Prob. 5SACh. 6 - Prob. 6SACh. 6 - Draw and label the four distinct phases of a...Ch. 6 - Prob. 8SACh. 6 - Prob. 9SACh. 6 - Prob. 10SACh. 6 - Explain the differences among photoautotrophs,...Ch. 6 - Prob. 12SACh. 6 - How does a chemostat maintain a constant...Ch. 6 - A scientist describes an organism as a...Ch. 6 - Pasteurization is a technique that uses...Ch. 6 - Prob. 3CTCh. 6 - Some organisms require riboflavin (vitamin B2) to...Ch. 6 - A scientist inoculates a bacterium into a complex...Ch. 6 - How can regions within biofilms differ in their...Ch. 6 - A scientific article describes a bacterium as an...Ch. 6 - Prob. 8CTCh. 6 - Prob. 9CTCh. 6 - Starting with 10 bacterial cells per milliliter in...Ch. 6 - Prob. 11CTCh. 6 - Prob. 12CTCh. 6 - The filamentous bacterium Beggiatoa gets its...Ch. 6 - Prob. 14CTCh. 6 - Examine the graph in Figure 6.3. Note that the...Ch. 6 - Prob. 16CTCh. 6 - Using the terms in Figure 6.8a, describe the...Ch. 6 - Prob. 18CTCh. 6 - Examine the ingredients of MacConkey agar as...Ch. 6 - Prob. 20CTCh. 6 - Using as many of the following terms as...Ch. 6 - Prob. 22CTCh. 6 - Viable plate counts are used to estimate...Ch. 6 - Using the following terms, fill in the following...Ch. 6 - Prob. 1TMWCh. 6 - Why do clinical laboratory scientists keep many...Ch. 6 - Prob. 3TMWCh. 6 - Culturing Microorganisms One of Kochs postulates...Ch. 6 - Clinical Case Study Boils in the Locker Room For...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- When viable cell concentrations are too high to count on an agar plate, it is common to use larger sized plates to increase the surface area for counting the colonies.True or falsearrow_forwardstarting with four bacterial cells per milliliter in a rich nutrient medium, with a 1-hour lag phase and a 30-minute generation time, how many cells will there be in 1 ml of this culture after 10 hours?arrow_forwardAssume an inoculum with a cell density of 108 cells per mL. The entire generation time takes 30 minutes. How many hours would it take to grow a culture to 108/mL if you started with a 10–2 dilution? helpful formula: g (generation time) = 0.301 (time)/ log x – log xoarrow_forward
- As shown in this diagram, you perform a ten-fold serial dilution of a culture to determine the number of colony forming units (CFU) per mL it contains. You do a plate count with these growth results (no. of colonies for each dilution): 1:10 too many to count; 1:100 too many to count; 1:1,000 312; 1:10,000 38; 1:100,000 no growth. The number of CFU per mL in the original culture was: A. 38 B. 380,000 C. 38,000 D. None of the other four answers (Correct answer not given) E. 10,000arrow_forwardAs shown in this diagram, you perform a ten-fold serial dilution of a culture to determine the number of colony forming units (CFU) per mL it contains. You do a plate count with these growth results (no. of colonies for each dilution): 1:10 too many to count; 1:100 too many to count; 1:1,000 174; 1:10,000 23; 1:100,000 no growth. The number of CFU per mL in the original culture was: 1 ml 1 ml Original inoculum Dilutions 9 ml broth in each tube 1:10 1 ml 174,000 1:100 1 ml 1 ml 1 ml 1:1000 1 ml 1:10,000 1 ml None of the other four answers (Correct answer not given) 1,000 230,000 174 1 ml 1:100,000 1 mlarrow_forwardA microbiologist has isolated a new strain of bacteria, and is trying to characterize aspects of its growth. Three flasks of the same media have different amounts of NaCl added. Other growth conditions are identical, and the flasks inoculated at the same time. At regular intervals over a 3 hour period, culture samples are taken and turbidity is measured. Here are the absorbance values over time in a table and graphed. Absorbance (OD600) Time (min) 20% NaCl 3% NaCl 0% NaCl 0 0.05 0.05 0.05 30 0.06 0.05 0.05 60 0.075 0.06 0.05 90 0.11 0.07 0.049 120 0.25 0.1 0.048 150 0.5 0.105 0.055 180 0.7 0.11 0.055 210 0.99 0.15 0.06 240 1.22 0.18 0.055 270 1.35 0.19 0.06 300 1.5 0.2 0.06 330 1.6 0.25 0.062 360 1.7 0.3 0.06 Based on these data, which of the following can be determined about this bacterium? It is psychrophilic. It…arrow_forward
- Below is a set of data for a serial dilution/plate count experiment. Based on this data what is the calculated concentration of bacteria in CFU/mL in the original culture? The OD600 of a bacterial culture that's been diluted in half is 0.842. What is the estimated population of cells in the original undiluted culture? asaparrow_forwardDescribe three problems associated with using the standard plate count method for determining the number of bacteria in a sample.arrow_forwardWhich is more Suitable for long-term storage: agar slant or plate? Explain why?arrow_forward
- Given the scenario, compute for the total volume of the culture media solution (milliliter or liter) and dehydrated media (grams). Scenario: The students of a Microbiology class were tasked to transfer or subculture a pure culture of Escherichia coli bacterium in five 7 mL nutrient broth and five petri dishes of nutrient agar with 20 mL capacity each. Based on the instruction bottles for nutrient broth and nutrient agar, preparation of the culture media is as follows. Nutrient broth: 8 g/liter Nutrient agar: 28 g/liter Formula: C1V1 = C2V2 *Concentration *Volume Computation: What are the answers to the following. Weight in grams of nutrient broth: _________ Distilled water in mL for nutrient broth: __________ Weight in grams of nutrient agar __________ Distilled water in mL for nutrient agar: ____________arrow_forwardUsing saline tubes containing 9 ml of saline (for dilutions), draw a diagram that would yield between 30 and 300 colonies from an original broth culture that contains 5 * 10^8 cells/ml. Indicate volumes used and dilutions made for each tube and plate.arrow_forwardAnother method of microbial control is filtration. List down at least 5 kinds of filters which could be used for this method?arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Serology 101: Testing for IgG and IgM antibodies; Author: Beckman Coulter Dx;https://www.youtube.com/watch?v=LtqKB-qpJrs;License: Standard youtube license