Biology
5th Edition
ISBN: 9781260487947
Author: BROOKER
Publisher: MCG
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Textbook Question
Chapter 5.1, Problem 1CS
Core Skill: Modeling The goal of this modeling challenge is to propose a model for a transmembrane protein.
Modeling Challenge: As shown in Figure 5.2, some α helices, which are called transmembrane a helices, may be inserted into the hydrophobic region of a membrane and span the entire membrane. Let’s suppose a protein in the plasma membrane has 5 transmembrane α helices. The loops that connect these 5 transmembrane segments are relatively short, except for the one that connects transmembrane segments 4 and 5, which is longer. The amino end projects into the cytosol. Draw a model of this transmembrane protein in the plasma membrane. In your model, draw the transmembrane a helices as cylinders and label them 1 through 5. Also label the amino and carboxyl ends and the cytosol and extracellular environment.
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Students have asked these similar questions
Multipass transmembrane proteins synthesized by ribosomes on the rough endoplasmic reticulum generally have which of the following arrangements of start-transfer and stop-transfer signals?
multiple start signals and multiple stop signals (to allow multiple transmembrane regions)
multiple start signals, but only one stop signal (to allow only one transmembrane region)
only one start signal, but multiple stop signals (to allow only one transmembrane region)
only one start signal, and only one stop signal (to allow only one transmembrane region)
only one stop signal, and only one start signal (to allow only one transmembrane region)
Example Problem: FRAP Data Interpretation
The diffusion rate of four different membrane proteins (A, B, C, and D) was measured using a FRAP
experiment with purified liposomes. The FRAP recovery curves are shown below.
Fluorescence
intensity
ROI
A
3
Time
B
Time
Post-bleaching
imaging
с
Time
D
Time
Pre-bleaching Bleaching
imaging
imaging
(a) Which membrane protein exhibits the higher rate of diffusion in the lipid bilayer? A or B? C or D?
(b) Explain the most likely cause of the difference in the recovery curves for proteins A and C.
Example Problem: FRAP Data Interpretation
The diffusion rate of four different membrane proteins (A, B, C, and D) was measured using a FRAP
experiment with purified liposomes. The FRAP recovery curves are shown below.
Fluorescence
intensity
ROI
A
3
Time
Pre-bleaching Bleaching
imaging
imaging
B
Time
Post-bleaching
imaging
C
Time
D
Time
(a) Which membrane protein exhibits the higher rate of diffusion in the lipid bilayer? A or B? C or D?
(b) Explain the most likely cause of the difference in the recovery curves for proteins A and C.
Chapter 5 Solutions
Biology
Ch. 5.1 - Core Skill: Modeling The goal of this modeling...Ch. 5.2 - Prob. 1CSCh. 5.3 - Prob. 1CCCh. 5.3 - Prob. 2CCCh. 5.4 - Prob. 1CSCh. 5.4 - Prob. 2CSCh. 5.4 - Lets suppose the inside of a cell has a solute...Ch. 5.5 - What is the purpose of gating?Ch. 5.5 - Prob. 1EQCh. 5.5 - Prob. 2EQ
Ch. 5.5 - Prob. 3EQCh. 5.6 - What is the function of the protein coat?Ch. 5 - Prob. 1TYCh. 5 - Which of the following events can never be...Ch. 5 - Lets suppose an insect, which doesnt maintain a...Ch. 5 - Prob. 4TYCh. 5 - Prob. 5TYCh. 5 - Prob. 6TYCh. 5 - Prob. 7TYCh. 5 - Prob. 8TYCh. 5 - Prob. 9TYCh. 5 - Large particles or large volumes of fluid can be...Ch. 5 - With your textbook closed, draw and describe the...Ch. 5 - Prob. 2CQCh. 5 - Prob. 3CQCh. 5 - Prob. 1COQCh. 5 - With regard to bringing solutes into the cell...
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- Exercise 1 Summarize the data below obtained from the protein experiments for determination of protein concentration in the form of a graph. Plot a graph containing a title and labels for both of the axis. Three readings were taken for each protein concentration. Find the averages and standard deviations for each reading and plot your graph using Microsoft EXCEL, complete with the error bars. This graph represents will be used to measure the protein concentration in an unknown protein solution. calibration curve for a protein assay (next experiment) where this Concentration of protein (Hg/mL) Absorbance, 595 nm 1 2 3 0.04 0.05 0.03 2 0.12 0.11 0.14 5 0.26 0.25 0.25 10 0.49 0.49 0.51 25 0.82 0.85 0.83 50 1.28 1.24 1.25arrow_forwardProblem: From the following information determine the amino acid sequence of a peptide. N-terminal Edman gives PTH-Alanine C terminal carboxypeptidase treatment, no observable reaction Trypsin cleavage gives three products Arg Peptide containing Ala, Lys Peptide containing Asp, Met, Phe, Pro Mild Chymotrypsin cleavage gives 2 peptides Peptide containing Asp, Pro Peptide containing Ala, Arg, Lys, Met, Phe CNBr cleavage gives 2 peptide Peptide containing Ala, Arg, Lys and homoserine Peptide containing Asp, Phe, Pro You must supply the answer as the 3-letter amino acid sequence from N-terminus to C-terminus in the form (you must use dashes, not spaces between the amino acids)arrow_forwardProblem: From the following information determine the amino acid sequence of a peptide. N-terminal Edman gives PTH-Alanine C terminal carboxypeptidase treatment, no observable reaction Trypsin cleavage gives three products Arg Peptide containing Ala, Lys Peptide containing Asp, Met, Phe, Pro Mild Chymotrypsin cleavage gives 2 peptides Peptide containing Asp, Pro Peptide containing Ala, Arg, Lys, Met, Phe CNBr cleavage gives 2 peptides Peptide containing Ala, Arg, Lys and homoserine Peptide containing Asp, Phe, Pro You must supply the answer as the 3-letter amino acid sequence from N-terminus to C-terminus in the form (you must use dashes, not spaces between the amino acids) Met-Thr-Glu-Trparrow_forward
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