Is the disk-diffusion technique measuring bacteriostatic or bactericidal activity? Briefly explain.

Question

Want to see more full solutions like this?

Answer 1

Textbook Question

Chapter 25, Problem 1Q

Is the disk-diffusion technique measuring bacteriostatic or bactericidal activity? Briefly explain.

Expert Solution & Answer

Summary Introduction

To write:

The nature of the activity of the disc-diffusion technique.

Introduction:

The disc-diffusion method is also called Kirby-Bauer test. It is a technique that tests the effectiveness of the antibiotics on a specific microorganism. An agar plate is spread with bacteria and then, paper discs of antibiotics are added to it. The bacteria are allowed to grow on the agar media and inhibition zones are observed.

Explanation of Solution

Antibiotics eliminate the bacteria completely. They are categorized as bactericidal, that is potent and bacteriostatic, which hampers growth. Bactericidal antibiotics kill the bacteria directly while the bacteriostatic antibiotics inhibit the bacteria from growing. The disc-diffusion technique works both by inhibiting the growth as well as by killing the bacteria.

The zone of inhibition is formed around the antibiotic discs. Bigger is the area, maximum will be the activity of the antibiotic. Similarly, lesser is the area, the minimum will be the activity of the antibiotic. Around the antibiotic discs, bacteria are not grown in any case nor the bacteria have been killed. They kill the bacteria as well as inhibit the growth also. They act both ways of the action.

Conclusion

Thus, disc-diffusion technique measured both the ways of bactericidal as well as bacteriostatic activity as they inhibit bacterial growth and killed the bacteria as well.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Students have asked these similar questions

In step 3 of today's protocol, it mentions that the incubation step with the P2 buffer should not be allowed to proceed for more than 5 minutes. a) What is the chemical composition and concentration (not mass) of each component of the P2 buffer?b) c) What is the purpose of adding the P2 buffer? What would be the major problem if this step is allowed to proceed for a long period of time?

You take 10 ml of a stock solution, which is at a concentration of 1000 phage/ml, and dilute it to a total of 100 ml. From the resulting solution you take 5 ml and dilute it to 25 ml, and from the latter you take 5 ml and make a total of 20 ml. a) It will be possible to know how many bacteriophage particles there will be in 1 ml of the last solution b) What is the dilution factor in each step, in the same order in which the dilutions are made? c) What is the total serial dilution factor?

The ribosome is the target for many important antibiotics. These drugs must discriminate between bacterial and eukaryotic ribosomes to achieve drug specificity and toxicity. For the two common antibiotics below, what is their mechanism of action and why are they more toxic to bacteria than eukaryotes? a) Tetracycline b) Erythromycin

Answer 2

Textbook Question

Chapter 25, Problem 1Q

Is the disk-diffusion technique measuring bacteriostatic or bactericidal activity? Briefly explain.

Expert Solution & Answer

Summary Introduction

To write:

The nature of the activity of the disc-diffusion technique.

Introduction:

The disc-diffusion method is also called Kirby-Bauer test. It is a technique that tests the effectiveness of the antibiotics on a specific microorganism. An agar plate is spread with bacteria and then, paper discs of antibiotics are added to it. The bacteria are allowed to grow on the agar media and inhibition zones are observed.

Explanation of Solution

Antibiotics eliminate the bacteria completely. They are categorized as bactericidal, that is potent and bacteriostatic, which hampers growth. Bactericidal antibiotics kill the bacteria directly while the bacteriostatic antibiotics inhibit the bacteria from growing. The disc-diffusion technique works both by inhibiting the growth as well as by killing the bacteria.

The zone of inhibition is formed around the antibiotic discs. Bigger is the area, maximum will be the activity of the antibiotic. Similarly, lesser is the area, the minimum will be the activity of the antibiotic. Around the antibiotic discs, bacteria are not grown in any case nor the bacteria have been killed. They kill the bacteria as well as inhibit the growth also. They act both ways of the action.

Conclusion

Thus, disc-diffusion technique measured both the ways of bactericidal as well as bacteriostatic activity as they inhibit bacterial growth and killed the bacteria as well.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 3

Textbook Question

Chapter 25, Problem 1Q

Is the disk-diffusion technique measuring bacteriostatic or bactericidal activity? Briefly explain.

Expert Solution & Answer

Summary Introduction

To write:

The nature of the activity of the disc-diffusion technique.

Introduction:

The disc-diffusion method is also called Kirby-Bauer test. It is a technique that tests the effectiveness of the antibiotics on a specific microorganism. An agar plate is spread with bacteria and then, paper discs of antibiotics are added to it. The bacteria are allowed to grow on the agar media and inhibition zones are observed.

Explanation of Solution

Antibiotics eliminate the bacteria completely. They are categorized as bactericidal, that is potent and bacteriostatic, which hampers growth. Bactericidal antibiotics kill the bacteria directly while the bacteriostatic antibiotics inhibit the bacteria from growing. The disc-diffusion technique works both by inhibiting the growth as well as by killing the bacteria.

The zone of inhibition is formed around the antibiotic discs. Bigger is the area, maximum will be the activity of the antibiotic. Similarly, lesser is the area, the minimum will be the activity of the antibiotic. Around the antibiotic discs, bacteria are not grown in any case nor the bacteria have been killed. They kill the bacteria as well as inhibit the growth also. They act both ways of the action.

Conclusion

Thus, disc-diffusion technique measured both the ways of bactericidal as well as bacteriostatic activity as they inhibit bacterial growth and killed the bacteria as well.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 4

Textbook Question

Chapter 25, Problem 1Q

Is the disk-diffusion technique measuring bacteriostatic or bactericidal activity? Briefly explain.

Expert Solution & Answer

Summary Introduction

To write:

The nature of the activity of the disc-diffusion technique.

Introduction:

The disc-diffusion method is also called Kirby-Bauer test. It is a technique that tests the effectiveness of the antibiotics on a specific microorganism. An agar plate is spread with bacteria and then, paper discs of antibiotics are added to it. The bacteria are allowed to grow on the agar media and inhibition zones are observed.

Explanation of Solution

Antibiotics eliminate the bacteria completely. They are categorized as bactericidal, that is potent and bacteriostatic, which hampers growth. Bactericidal antibiotics kill the bacteria directly while the bacteriostatic antibiotics inhibit the bacteria from growing. The disc-diffusion technique works both by inhibiting the growth as well as by killing the bacteria.

The zone of inhibition is formed around the antibiotic discs. Bigger is the area, maximum will be the activity of the antibiotic. Similarly, lesser is the area, the minimum will be the activity of the antibiotic. Around the antibiotic discs, bacteria are not grown in any case nor the bacteria have been killed. They kill the bacteria as well as inhibit the growth also. They act both ways of the action.

Conclusion

Thus, disc-diffusion technique measured both the ways of bactericidal as well as bacteriostatic activity as they inhibit bacterial growth and killed the bacteria as well.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 5

Textbook Question

Answer 6

Textbook Question

Answer 7

Expert Solution & Answer

Summary Introduction

To write:

The nature of the activity of the disc-diffusion technique.

Introduction:

The disc-diffusion method is also called Kirby-Bauer test. It is a technique that tests the effectiveness of the antibiotics on a specific microorganism. An agar plate is spread with bacteria and then, paper discs of antibiotics are added to it. The bacteria are allowed to grow on the agar media and inhibition zones are observed.

Explanation of Solution

Antibiotics eliminate the bacteria completely. They are categorized as bactericidal, that is potent and bacteriostatic, which hampers growth. Bactericidal antibiotics kill the bacteria directly while the bacteriostatic antibiotics inhibit the bacteria from growing. The disc-diffusion technique works both by inhibiting the growth as well as by killing the bacteria.

The zone of inhibition is formed around the antibiotic discs. Bigger is the area, maximum will be the activity of the antibiotic. Similarly, lesser is the area, the minimum will be the activity of the antibiotic. Around the antibiotic discs, bacteria are not grown in any case nor the bacteria have been killed. They kill the bacteria as well as inhibit the growth also. They act both ways of the action.

Conclusion

Thus, disc-diffusion technique measured both the ways of bactericidal as well as bacteriostatic activity as they inhibit bacterial growth and killed the bacteria as well.

Answer 8

Expert Solution & Answer

Summary Introduction

To write:

The nature of the activity of the disc-diffusion technique.

Introduction:

The disc-diffusion method is also called Kirby-Bauer test. It is a technique that tests the effectiveness of the antibiotics on a specific microorganism. An agar plate is spread with bacteria and then, paper discs of antibiotics are added to it. The bacteria are allowed to grow on the agar media and inhibition zones are observed.

Explanation of Solution

Antibiotics eliminate the bacteria completely. They are categorized as bactericidal, that is potent and bacteriostatic, which hampers growth. Bactericidal antibiotics kill the bacteria directly while the bacteriostatic antibiotics inhibit the bacteria from growing. The disc-diffusion technique works both by inhibiting the growth as well as by killing the bacteria.

The zone of inhibition is formed around the antibiotic discs. Bigger is the area, maximum will be the activity of the antibiotic. Similarly, lesser is the area, the minimum will be the activity of the antibiotic. Around the antibiotic discs, bacteria are not grown in any case nor the bacteria have been killed. They kill the bacteria as well as inhibit the growth also. They act both ways of the action.

Conclusion

Thus, disc-diffusion technique measured both the ways of bactericidal as well as bacteriostatic activity as they inhibit bacterial growth and killed the bacteria as well.

Answer 9

Expert Solution & Answer

Answer 10

Expert Solution & Answer

Answer 11

Summary Introduction

To write:

The nature of the activity of the disc-diffusion technique.

Introduction:

The disc-diffusion method is also called Kirby-Bauer test. It is a technique that tests the effectiveness of the antibiotics on a specific microorganism. An agar plate is spread with bacteria and then, paper discs of antibiotics are added to it. The bacteria are allowed to grow on the agar media and inhibition zones are observed.

Answer 12

Explanation of Solution

Antibiotics eliminate the bacteria completely. They are categorized as bactericidal, that is potent and bacteriostatic, which hampers growth. Bactericidal antibiotics kill the bacteria directly while the bacteriostatic antibiotics inhibit the bacteria from growing. The disc-diffusion technique works both by inhibiting the growth as well as by killing the bacteria.

The zone of inhibition is formed around the antibiotic discs. Bigger is the area, maximum will be the activity of the antibiotic. Similarly, lesser is the area, the minimum will be the activity of the antibiotic. Around the antibiotic discs, bacteria are not grown in any case nor the bacteria have been killed. They kill the bacteria as well as inhibit the growth also. They act both ways of the action.

Answer 13

Conclusion

Thus, disc-diffusion technique measured both the ways of bactericidal as well as bacteriostatic activity as they inhibit bacterial growth and killed the bacteria as well.

Answer 14

Ch. 25 - Is the disk-diffusion technique measuring...Ch. 25 - Prob. 2Q Ch. 25 - Why is the disk-diffusion technique not a perfect...Ch. 25 - What effect would the presence of tetracycline in...Ch. 25 - The following results were obtained from a...

Is the disk-diffusion technique measuring bacteriostatic or bactericidal activity? Briefly explain.

Videos

Explanation of Solution

Want to see more full solutions like this?

Chapter 25 Solutions