Biochemistry
6th Edition
ISBN: 9781305577206
Author: Reginald H. Garrett, Charles M. Grisham
Publisher: Cengage Learning
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Chapter 23, Problem 16P
Extending the Mechanism of Methylmalonyl-CoA Mutase to Similar Reactions Based on the mechanism for the methylmalonyl-CoA mutase (see problem 14), write reasonable mechanisms for the following reactions shown.
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Compare and contrast Pyruvate Dehydrogenase with a-ketoglutarate dehydrogenaseOutline the mechanisms of both enzymes. Discuss the functions of the coenzymes. List the similarities and the differences between the 2 enzymes. Both are very large membrane bound complexes. What are the advantages of this strategy?How detailed is the enzyme structure known below(It's Pyruvate Dehydrogenase )? What insight(s) does this structural detail give you about the enzyme mechanism.
25. The AG" values for the two reactions are given.
1.
2.
oxaloacetate + acetyl-CoA + H₂O → citrate + COASH
oxaloacetate + acetate → citrate
Enzymes for reactions 1 and 2 are citrate synthase and citrate lyase, respectively.
Determine the AG" for the hydrolysis of acetyl-CoA
acetyl-CoA + H₂O
acetate + COASH + H+
plane een isoimorfbold bns
(94) opg
Vp1903 9911
AG¹⁰ ?
AGO = -32.2 kJ/mol
AG"=-1.9 kJ/mol
Provide a reasonable step-wise mechanism for the reaction below, involving FAD as a coenzyme and invoking any general acids and bases from acyl-CoA dehydrogenase that you might need (just draw the tricyclic flavin core of FAD; no need to show the ribitol or ADP
Chapter 23 Solutions
Biochemistry
Ch. 23 - Prob. 1PCh. 23 - Determining the Amount of ATP Produced from Fatty...Ch. 23 - Prob. 3PCh. 23 - Examining the Labeling of Glucose from 14C.labeled...Ch. 23 - Prob. 5PCh. 23 - Prob. 6PCh. 23 - Prob. 7PCh. 23 - Prob. 8PCh. 23 - Prob. 9PCh. 23 - Prob. 10P
Ch. 23 - Prob. 11PCh. 23 - Understanding Human Energy Consumption During...Ch. 23 - Prob. 13PCh. 23 - Prob. 14PCh. 23 - Prob. 15PCh. 23 - Extending the Mechanism of Methylmalonyl-CoA...Ch. 23 - Prob. 17PCh. 23 - Prob. 18PCh. 23 - Prob. 19PCh. 23 - Understanding a Ubiquitous Series of Metabolic...Ch. 23 - Using the ActiveModel for enoyl-CoA dehydratase,...
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- Several pathways exist in different organisms for the metabolism of propionyl-CoA. Most organisms utilizethe pathway that feeds into the citric acid cycle via succinyl-CoA. Another metabolic route used by Clostridium kluyveriis a "continuation of -oxidation" and consists of the following enzymatic reactions: (a) propionyl-CoA is converted toacryloyl-CoA; (b) formation of 3-hydroxypropionyl-CoA; (c) conversion to malonyl semialdehyde CoA; (d) productionof malonyl-CoA, which is then (e) decarboxylated to produce acetyl-CoA. Outline the above steps between propionylCoA and acetyl-CoA. Show structures for the metabolites and indicate appropriate cofactors (free or bound) that may beinvolved.arrow_forwardSeveral pathways exist in different organisms for the metabolism of propionyl-CoA. Most organisms utilizethe pathway that feeds into the citric acid cycle via succinyl-CoA. Another metabolic route used by Clostridium kluyveriis a "continuation of -oxidation" and consists of the following enzymatic reactions: (a) propionyl-CoA is converted toacryloyl-CoA; (b) formation of 3-hydroxypropionyl-CoA; (c) conversion to malonyl semialdehyde CoA; (d) productionof malonyl-CoA, which is then (e) decarboxylated to produce acetyl-CoA. Outline the above steps between propionylCoA and acetyl-CoA. Show structures for the metabolites and indicate appropriate cofactors (free or bound) that may beinvolved. b) Suggest a mechanism for enzyme reaction (e).arrow_forwardConsider the complete oxidation of one mole of the fatty acyl CoA of myristic acid (14:0). Determine the following (Type the numerical value only - e.g. 1): 1. rounds of B-oxidation 2. total no. of acetyl CoA produced 3. total no. of NADH produced from all rounds of B-oxidation 4. total no. of FADH2 produced from all rounds of B-oxidation 5. total no. of NADH produced from the oxidation of all acetyl CoA in the TCA cycle 6. total no. of FADH, produced from the oxidation of all acetyl CoA in the TCA cycle 7. total ATP yieldarrow_forward
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