Concept explainers
(a)
To determine: The type of reaction in which leucine is converted to α-ketoisocaproate.
Introduction:
The series of
β-oxidation is the oxidation of fatty acids in cells in absence of glucose and glycogen, to produce ATP. Oxidation takes place at the β carbon, thus, this reaction is termed as β-oxidation.
(a)
Explanation of Solution
Pictorial representation: Fig. 1 represents the transamination reaction.
Fig. 1: Transamination reaction.
Explanation:
The transamination reaction is involved in the conversion of leucine into alpha ketoisocaproate. The co-factor involved in this reaction is PLP (pyridoxal phosphate). There is no analogous reaction to this reaction from citric acid cycle and β-oxidation.
(b)
To determine: The type of reaction in which Ketoisocaproate is converted to Isovaleryl-CoA.
Introduction:
The series of metabolic reactions, in which the energy stored in the compounds is released for the production of ATP (adenosine triphosphate), is called “Krebs’s cycle”. The pyruvate undergoes series of reactions in the citric acid cycle and converts into Acetyl CoA (acetyl coenzyme A) in the presence of oxygen. β-oxidation is the oxidation of fatty acids in cells in absence of glucose and glycogen, to produce ATP. Oxidation takes place at the β carbon, thus, this reaction is termed as β-oxidation.
(b)
Explanation of Solution
Pictorial representation: Fig. 2 represents the oxidative decarboxylation.
Fig. 2: Oxidative decarboxylation.
Explanation:
The oxidative decarboxylation is occurring that converts Ketoisocaproate to Isovaleryl-CoA. The cofactors involved are “NAD+ (Nicotinamide adenine dinucleotide)”, “TPP (Triphenyl phosphate)” and “FAD (Flavin adenine dinucleotide)”, and “lipoate”. The formation of acetyl CoA by oxidative decarboxylation of pyruvate is similar to this given reaction.
(c)
To determine: The type of reaction in which Isovaleryl-CoA is converted to β-methylcrotonyl-CoA.
Introduction:
The series of metabolic reactions, in which the energy stored in the compounds is released for the production of ATP (adenosine triphosphate), is called “Krebs’s cycle”. The pyruvate undergoes series of reactions in the citric acid cycle and converts into Acetyl CoA (acetyl coenzyme A) in the presence of oxygen.
β-oxidation is the oxidation of fatty acids in cells in absence of glucose and glycogen, to produce ATP. Oxidation takes place at the β carbon, thus, this reaction is termed as β-oxidation.
(c)
Explanation of Solution
Pictorial representation: Fig. 3 represents the dehydrogenation reaction.
Fig. 3: Dehydrogenation reaction.
Explanation:
The type of reaction in which Isovaleryl-CoA is converted to β-methylcrotonyl-CoA is dehydrogenation. The cofactor is (FAD) Flavin adenine
(d)
To determine: The type of reaction in which beta methylcrotonyl-CoA is converted to beta-methylglutaconyl-CoA.
Introduction:
The series of metabolic reactions, in which the energy stored in the compounds is released for the production of ATP (adenosine triphosphate), is called “Krebs’s cycle”. The pyruvate undergoes series of reactions in the citric acid cycle and converts into Acetyl CoA (acetyl coenzyme A) in the presence of oxygen. β-oxidation is the oxidation of fatty acids in cells in absence of glucose and glycogen, to produce ATP. Oxidation takes place at the β carbon, thus, this reaction is termed as β-oxidation.
(d)
Explanation of Solution
Pictorial representation: Fig. 4 represents the carboxylation reaction.
Fig. 4: Carboxylation reaction.
Explanation:
The formation of β-methylglutaconyl-CoA by β-methylcrotonyl-CoA is a type of carboxylation reaction. The cofactors are biotin and ATP. This step is not analogous to any step in β-oxidation and citric acid cycle.
(e)
To determine: The type of reaction in which beta-methylglutaconyl-CoA is converted to β-hydroxy β-methylglutaryl-CoA.
Introduction:
The series of metabolic reactions, in which the energy stored in the compounds is released for the production of ATP (adenosine triphosphate), is called “Krebs’s cycle”. The pyruvate undergoes series of reactions in the citric acid cycle and converts into Acetyl CoA (acetyl coenzyme A) in the presence of oxygen. β-oxidation is the oxidation of fatty acids in cells in absence of glucose and glycogen, to produce ATP. Oxidation takes place at the β carbon, thus, this reaction is termed as β-oxidation.
(e)
Explanation of Solution
Pictorial representation: Fig. 5 represents the hydration reaction.
Fig. 5: Hydration reaction.
Explanation:
The conversion of beta-methylglutaconyl-CoA into β-hydroxy β-methylglutaryl-CoA is a type of hydration reaction. This reaction does not require the cofactors. The formation of malate by fumarate and formation of 3-hydroxyacyl-CoA by enoyl-CoA are analogous to this given reaction.
(f)
To determine: The type of reaction in which β-hydroxy β-methylglutaryl-CoA is converted to acetyl-CoA and acetoacetate.
Introduction:
The series of metabolic reactions, in which the energy stored in the compounds is released for the production of ATP (adenosine triphosphate), is called “Krebs’s cycle”. The pyruvate undergoes series of reactions in the citric acid cycle and converts into Acetyl CoA (acetyl coenzyme A) in the presence of oxygen.
β-oxidation is the oxidation of fatty acids in cells in absence of glucose and glycogen, to produce ATP. Oxidation takes place at the β carbon, thus, this reaction is termed as β-oxidation.
(f)
Explanation of Solution
Pictorial representation: Fig. 6 represents the reverse aldol reaction.
Fig. 6: Reverse aldol reaction.
Explanation:
The formation of acetate-CoA and acetoacetate by β-hydroxy β-methylglutaryl-CoA is a type of reverse aldol reaction. There are no cofactors involved in this reaction. The analogous reaction is the opposite reaction involving citrate synthase. This reaction is analogous to citrate synthase reaction in the citric acid cycle. It is identical to the cleavage of β-hydroxy-β-methylglutaryl-CoA in the formation of
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