Laboratory Experiments in Microbiology (11th Edition)
11th Edition
ISBN: 9780321994936
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Textbook Question
Chapter 14, Problem 2CT
How could you determine which of the following carbohydrates a bacterium fermented: mannitol, sorbitol, adonitol, or arabinose?
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Provide the conditions (e.g., intrinsic properties, storage conditions, etc.) that will make the recovery of the following microorganisms in commercially sterile foods acceptable:
a) Obligate thermophilic sporeforming bacteria
b) Acid-tolerant microorganisms
c) Mesophilic or thermophilic sporeformers
You are given a mixed culture of 3 bacteria with following characteristics-
1) Bacteria 1 - Methicillin- resistant Staphylococcus aureus (MRSA) (does not ferment any of these 4
sugars but forms endospores and is sensitive to penicillin)
2) Bacteria 2 - Escherichia coli (can ferment glucose, maltose, sucrose and lactose) and also produce
carbon dioxide gas on fermenting these
3) Bacteria 3 - Enterobacter aerogenes. (can ferment only sucrose and cannot ferment glucose, maltose
and lactose. Also on fermenting sucrose it produces hydrogen sulphide gas.)
Also they all give similar looking white-colored colonies on an agar plate.
Question: Generate separate pure cultures of these 3 bacteria from the mixed culture using the following tools
that you learned in the lab. Use any (some or all) of these tools to generate pure cultures as well as
confirm them. Any other technique that you use should be one you learned in lab. No need to give
detailed info about how to perform the technique but it…
If the result of an unknown bacteria is “poor growth” and “red growth,” can this result be interpreted as “organism does not ferment mannitol?” Explain why or why not.
If the result of an unknown bacteria is “good growth” and “yellow growth,” is the unknown bacteria more likely Staphylococcus aureus or Staphylococcus epidermidis?
Chapter 14 Solutions
Laboratory Experiments in Microbiology (11th Edition)
Ch. 14 - Why are fermentation tubes evaluated at 24 and 48...Ch. 14 - If an organism metabolizes glucose aerobically,...Ch. 14 - Which of these media is selective?...Ch. 14 - Could an organism be a fermenter and also be both...Ch. 14 - How could you determine which of the following...Ch. 14 - If a bacterium cannot ferment glucose, why not...Ch. 14 - If a bacterium cannot use glucose, what result...Ch. 14 - If a bacterium cannot use citrate, what result...Ch. 14 - You put an opened bottle of orange drink in the...
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