Biology
Biology
12th Edition
ISBN: 9780134813448
Author: Audesirk, Teresa, Gerald, Byers, Bruce E.
Publisher: Pearson,
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Chapter 14, Problem 1MC
Summary Introduction

Introduction: Deoxyribonucleic acid (DNA) is the hereditary material that contains the essential genetic information of an organism. The defects that occur in the DNA can be rectified by various methods of biotechnology such as DNA sequencing, polymerase chain reaction (PCR), and genetic engineering.

Expert Solution & Answer
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Answer to Problem 1MC

Correct answer: Option a. “produces many copies of specific DNA sequences.”

Explanation of Solution

Reason for the correct answer:

Option a. is given as “produces many copies of specific DNA sequences.”

The polymerase chain reaction (PCR) is the maximum used method for manipulating DNA. Specific amplification of particular DNA sequence produces numerous copies of desirable DNA sequences in this method.

Hence, option a. is correct.

Reasons for incorrect answers:

Option b. is given as “is a method for sequencing DNA.”

DNA sequence is the method of determining the nucleic acid sequence based on the order of the nucleotides whereas, PCR is the method of artificial DNA manipulation

Hence, option b. is incorrect.

Option c. is given as “is a technique for editing a genome.”

Genome editing is the process of deleting, replacing, or inserting a DNA sequence in order to manipulate the genome of an organism. The PCR is not a technique for genome editing.

Hence, option c. is incorrect.

Option d. is given as “is used to determine whether a specific gene is expressed.”

Gene expression is determined by microarrays and targeted RNA and it cannot be determined using the PCR.

Hence, option d. is incorrect.

Hence, options b., c., and d. are incorrect.

Conclusion

Numerous copies of specific DNA sequences are produced using the PCR.

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Students have asked these similar questions
Transcriptomes, that is, all the transcripts in a cell, are analyzed using:     a. a type of PCR   b. fluorescently labeled nucleotides    c. microarrays   d. all the above
When constructing a recombinant DNA molecule, a marker gene is used to:   a. give the organism a new trait, such as insect resistance   b. Identify whether the transformed organism contains the recombinant DNA   c. replicate (copy) the gene of interest   d. Introduce the recombinant DNA into an organism   e. cut short sequences of DNA
Place the steps of sanger sequencing in order.A.  A fluorescent laser excites the fragments and records the wavelength consistent with a single nucleotide. B.  ddNTPs bind and stop chain extension.C.  DNA fragments are separated by size through a capillary tube. D.  DNA polymerase copies the target region of template DNA.E.  The final nucleotide of each fragment is labeled with a fluorescent tag.

Chapter 14 Solutions

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