Microbiology: An Introduction
11th Edition
ISBN: 9780321733603
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: Benjamin Cummings
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Textbook Question
Chapter 12, Problem 2R
A mixed culture of Escherichia coli and Penicillium chrysogenum is inoculated onto the following culture media. On which medium would you expect each to grow? Why?
- a. 0.5% peptone in tap water
- b. 10% glucose in tap water
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After inoculating and incubating an agar slant from a pure broth culture of a bacterial species such as E. coli, which of the following would indicate an unsuccessful aseptic transfer? (Choose ALL that apply)
a - There is fungal growth in the original broth culture tube.
b- There is too much growth on the agar slant.
c- There are colonies of similar morphology on the slant.
d - There are red, yellow, and white colonies on the slant.
e - There is no growth on the slant.
A. Antibiotics, such as gentamicin, are used in media to prevent growth of susceptible microbes. Is gentamicin media a selective, differential or enrichment media?
B. Sorbitol is a sugar alcohol that all Escherichia bacteria can normally ferment into an acidic waste compound. This acidic end product is detected by including a pH indicator in the agar and noticing a color change. The strain of Escherichia that is a common food pathogen, E. coli O157:H7, however can not ferment sorbitol. It will grow similar to other E. coli, but not change the agar color. Is sorbitol in nutrient agar a selective, differential or enrichment media?
Chapter 12 Solutions
Microbiology: An Introduction
Ch. 12 - Prob. 1RCh. 12 - A mixed culture of Escherichia coli and...Ch. 12 - Prob. 3RCh. 12 - Prob. 4RCh. 12 - Differentiate cellular and plasmodial slime molds....Ch. 12 - Prob. 6RCh. 12 - Prob. 7RCh. 12 - Prob. 8RCh. 12 - Prob. 9RCh. 12 - DRAW IT A generalized life cycle of the liver...
Ch. 12 - How many phyla are represented in the following...Ch. 12 - Prob. 2MCQCh. 12 - Prob. 3MCQCh. 12 - Fleas are the intermediate host for Dioylium...Ch. 12 - Prob. 5MCQCh. 12 - Prob. 6MCQCh. 12 - The definitive host for Plasmodium vivax is a....Ch. 12 - Prob. 8MCQCh. 12 - Prob. 9MCQCh. 12 - Prob. 10MCQCh. 12 - Alexandrium (red tide) has been called a plant...Ch. 12 - The life cycle of the fish tapeworm...Ch. 12 - Trypanosoma brucei gambiense-part (a) in the...Ch. 12 - Prob. 1CAECh. 12 - Prob. 2CAECh. 12 - A teenaged male in California complained of...
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- E.coli was incubated with aeration in a nutrient medium containing two carbon sources provided one at a time, and the following growth curve was made from this culture. a. Explain what happened at the time marked x. b. Which substrate provided “better” growth conditions for the bacteria? How can you tell?arrow_forwardA culture of S. cerevisea has an overnight OD of 4.5 (1.0 OD is approx 1.0x107 cells/ml) You will be plating 100µl onto agar and want the final count of colonies on the plate to be around 300 colonies. How much of the 4.5 OD culture must you use to get a 500µl subdilution (with sterile water), so that you have diluted enough to get approx 300 colonies per 100ularrow_forwardAhmad has prepared a seed culture containing recombinant Escherichia coli for the production of lipase enzyme. The seed culture was incubated for 18 hours before inoculating 5% of the seed culture into 2L of production media. a. Predict the consequences if the seed culture has been contaminated. b. Discuss whether the seed culture is meeting the criteria as inoculum or not. asap please.arrow_forward
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