Biology
Biology
5th Edition
ISBN: 9781260487947
Author: BROOKER
Publisher: MCG
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Chapter 11.1, Problem 1CS
Summary Introduction

To explain: The way in which bacterial transformation plays a role in the transfer of genes, such as antibiotic resistance genes, from one bacterial species to another.

Introduction: Bacteria are unicellular organisms that lack a well-defined nuclear membrane. Bacteria are classified into various categories on the basis of their size, shape, habitat, and functions. They are capable of causing severe infections and disorders in humans. The science that deals with a large-scale study of bacteria are termed as bacteriology

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Results from a Kirby Bauer antibiotic assay on a Gram-negative bacterial culture are described as follows: A) the bacterium is resistant to penicillin, an antibiotic that targets synthesis of the peptidoglycan cell wall and B) the bacterium is resistant to tetracycline, an antibiotic that targets the small subunit of the ribosome, inhibiting protein synthesis. Which of the results represents intrinsic resistant and which represents acquired resistants?
Explain:  Describe an electrochemical sensor assay method for rapid bacterial detection and identification. What are the principles and mechanisms involved? functionalization of a sensor array with DNA oligonucleotide capture probes for ribosomal RNA (rRNA) species-specific sequences.  sandwich hybridization of target rRNA with the capture probe and a horseradish peroxidase linked DNA oligonucleotide detector probe. Explain how bacteria are being detected using an electrochemical sensor
How is bacterial transformation used in biotechnology and How can we tell if we have successful transaction in bacterial transformation?
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