A constitutive mutant is a strain that continuously makes a protein that is inducible in the wild type. Describe two ways in which a change in a DNA molecule could lead to the emergence of a constitutive mutant. How could these two types of constitutive mutants be distinguished genetically?

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Answer 1

Textbook Question

Chapter 11, Problem 1AQ

A constitutive mutant is a strain that continuously makes a protein that is inducible in the wild type. Describe two ways in which a change in a DNA molecule could lead to the emergence of a constitutive mutant. How could these two types of constitutive mutants be distinguished genetically?

Expert Solution & Answer

Summary Introduction

To discuss:

A constitutive mutant (a strain) can continuously produce a protein molecule that is inducible in the wild type. Describe two ways to produce a constitutive mutant through DNA change. How these two types of constitutive mutants are differentiated genetically.

Concept introduction:

A mutant strain can continuously synthesize a protein because of a mutation in the regulatory gene is known as a constitutive mutant. The regulatory gene is always expressed or not switched off. A strain with a mutation in the lac operon is an example of a constitutive mutant. The lac operon mutation can lead to the transcription of the lac genes in the absence of lactose in the medium.

Explanation of Solution

Formation of a constitutive mutant:

A constitutive mutant can be produced if the altered repressor protein not able to recognize the operator due to mutation.
A constitutive mutant can be produced if an operator locus not able to interact with the normal repressor due to mutation.

In both cases, the mutation can lead to continuous production of the protein. The two constitutive mutants can be differentiated genetically by the cis-trans test or a complementation test in merodiploid cells. This test is used to find whether the mutations found in the two mutant strains are in different genes or not.

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Answer 2

Textbook Question

Chapter 11, Problem 1AQ

A constitutive mutant is a strain that continuously makes a protein that is inducible in the wild type. Describe two ways in which a change in a DNA molecule could lead to the emergence of a constitutive mutant. How could these two types of constitutive mutants be distinguished genetically?

Expert Solution & Answer

Summary Introduction

To discuss:

A constitutive mutant (a strain) can continuously produce a protein molecule that is inducible in the wild type. Describe two ways to produce a constitutive mutant through DNA change. How these two types of constitutive mutants are differentiated genetically.

Concept introduction:

A mutant strain can continuously synthesize a protein because of a mutation in the regulatory gene is known as a constitutive mutant. The regulatory gene is always expressed or not switched off. A strain with a mutation in the lac operon is an example of a constitutive mutant. The lac operon mutation can lead to the transcription of the lac genes in the absence of lactose in the medium.

Explanation of Solution

Formation of a constitutive mutant:

A constitutive mutant can be produced if the altered repressor protein not able to recognize the operator due to mutation.
A constitutive mutant can be produced if an operator locus not able to interact with the normal repressor due to mutation.

In both cases, the mutation can lead to continuous production of the protein. The two constitutive mutants can be differentiated genetically by the cis-trans test or a complementation test in merodiploid cells. This test is used to find whether the mutations found in the two mutant strains are in different genes or not.

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Answer 3

Textbook Question

Chapter 11, Problem 1AQ

A constitutive mutant is a strain that continuously makes a protein that is inducible in the wild type. Describe two ways in which a change in a DNA molecule could lead to the emergence of a constitutive mutant. How could these two types of constitutive mutants be distinguished genetically?

Expert Solution & Answer

Summary Introduction

To discuss:

A constitutive mutant (a strain) can continuously produce a protein molecule that is inducible in the wild type. Describe two ways to produce a constitutive mutant through DNA change. How these two types of constitutive mutants are differentiated genetically.

Concept introduction:

A mutant strain can continuously synthesize a protein because of a mutation in the regulatory gene is known as a constitutive mutant. The regulatory gene is always expressed or not switched off. A strain with a mutation in the lac operon is an example of a constitutive mutant. The lac operon mutation can lead to the transcription of the lac genes in the absence of lactose in the medium.

Explanation of Solution

Formation of a constitutive mutant:

A constitutive mutant can be produced if the altered repressor protein not able to recognize the operator due to mutation.
A constitutive mutant can be produced if an operator locus not able to interact with the normal repressor due to mutation.

In both cases, the mutation can lead to continuous production of the protein. The two constitutive mutants can be differentiated genetically by the cis-trans test or a complementation test in merodiploid cells. This test is used to find whether the mutations found in the two mutant strains are in different genes or not.

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Answer 4

Textbook Question

Chapter 11, Problem 1AQ

A constitutive mutant is a strain that continuously makes a protein that is inducible in the wild type. Describe two ways in which a change in a DNA molecule could lead to the emergence of a constitutive mutant. How could these two types of constitutive mutants be distinguished genetically?

Expert Solution & Answer

Summary Introduction

To discuss:

A constitutive mutant (a strain) can continuously produce a protein molecule that is inducible in the wild type. Describe two ways to produce a constitutive mutant through DNA change. How these two types of constitutive mutants are differentiated genetically.

Concept introduction:

A mutant strain can continuously synthesize a protein because of a mutation in the regulatory gene is known as a constitutive mutant. The regulatory gene is always expressed or not switched off. A strain with a mutation in the lac operon is an example of a constitutive mutant. The lac operon mutation can lead to the transcription of the lac genes in the absence of lactose in the medium.

Explanation of Solution

Formation of a constitutive mutant:

A constitutive mutant can be produced if the altered repressor protein not able to recognize the operator due to mutation.
A constitutive mutant can be produced if an operator locus not able to interact with the normal repressor due to mutation.

In both cases, the mutation can lead to continuous production of the protein. The two constitutive mutants can be differentiated genetically by the cis-trans test or a complementation test in merodiploid cells. This test is used to find whether the mutations found in the two mutant strains are in different genes or not.

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Answer 5

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Answer 6

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Answer 7

Expert Solution & Answer

Summary Introduction

To discuss:

A constitutive mutant (a strain) can continuously produce a protein molecule that is inducible in the wild type. Describe two ways to produce a constitutive mutant through DNA change. How these two types of constitutive mutants are differentiated genetically.

Concept introduction:

A mutant strain can continuously synthesize a protein because of a mutation in the regulatory gene is known as a constitutive mutant. The regulatory gene is always expressed or not switched off. A strain with a mutation in the lac operon is an example of a constitutive mutant. The lac operon mutation can lead to the transcription of the lac genes in the absence of lactose in the medium.

Explanation of Solution

Formation of a constitutive mutant:

A constitutive mutant can be produced if the altered repressor protein not able to recognize the operator due to mutation.
A constitutive mutant can be produced if an operator locus not able to interact with the normal repressor due to mutation.

In both cases, the mutation can lead to continuous production of the protein. The two constitutive mutants can be differentiated genetically by the cis-trans test or a complementation test in merodiploid cells. This test is used to find whether the mutations found in the two mutant strains are in different genes or not.

Answer 8

Expert Solution & Answer

Summary Introduction

To discuss:

A constitutive mutant (a strain) can continuously produce a protein molecule that is inducible in the wild type. Describe two ways to produce a constitutive mutant through DNA change. How these two types of constitutive mutants are differentiated genetically.

Concept introduction:

A mutant strain can continuously synthesize a protein because of a mutation in the regulatory gene is known as a constitutive mutant. The regulatory gene is always expressed or not switched off. A strain with a mutation in the lac operon is an example of a constitutive mutant. The lac operon mutation can lead to the transcription of the lac genes in the absence of lactose in the medium.

Explanation of Solution

Formation of a constitutive mutant:

A constitutive mutant can be produced if the altered repressor protein not able to recognize the operator due to mutation.
A constitutive mutant can be produced if an operator locus not able to interact with the normal repressor due to mutation.

In both cases, the mutation can lead to continuous production of the protein. The two constitutive mutants can be differentiated genetically by the cis-trans test or a complementation test in merodiploid cells. This test is used to find whether the mutations found in the two mutant strains are in different genes or not.

Answer 9

Expert Solution & Answer

Answer 10

Expert Solution & Answer

Answer 11

Summary Introduction

To discuss:

A constitutive mutant (a strain) can continuously produce a protein molecule that is inducible in the wild type. Describe two ways to produce a constitutive mutant through DNA change. How these two types of constitutive mutants are differentiated genetically.

Concept introduction:

A mutant strain can continuously synthesize a protein because of a mutation in the regulatory gene is known as a constitutive mutant. The regulatory gene is always expressed or not switched off. A strain with a mutation in the lac operon is an example of a constitutive mutant. The lac operon mutation can lead to the transcription of the lac genes in the absence of lactose in the medium.

Answer 12

Explanation of Solution

Formation of a constitutive mutant:

A constitutive mutant can be produced if the altered repressor protein not able to recognize the operator due to mutation.
A constitutive mutant can be produced if an operator locus not able to interact with the normal repressor due to mutation.

In both cases, the mutation can lead to continuous production of the protein. The two constitutive mutants can be differentiated genetically by the cis-trans test or a complementation test in merodiploid cells. This test is used to find whether the mutations found in the two mutant strains are in different genes or not.

Answer 13

Ch. 11.1 - Distinguish between a mutation and a mutant.Ch. 11.1 - Distinguish between screening and selection.Ch. 11.1 - Prob. 3MQ Ch. 11.1 - Write a one-sentence definition of the term...Ch. 11.2 - Do missense mutations occur in genes encoding...Ch. 11.2 - Why do frameshift mutations generally have more...Ch. 11.2 - Prob. 1CR Ch. 11.3 - Why are suppressor tRNA mutations not lethal?Ch. 11.3 - Which class of mutation, missense or nonsense, is...Ch. 11.3 - What is the difference between same-site and...

A constitutive mutant is a strain that continuously makes a protein that is inducible in the wild type. Describe two ways in which a change in a DNA molecule could lead to the emergence of a constitutive mutant. How could these two types of constitutive mutants be distinguished genetically?

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