Interpretation:
In the preparation of a TLC plate for the development, the result of making large spots needs to be explained.
Concept Introduction:
Chromatography is the best method of separation of components from the mixture as it gives most accurate results compare to other methods. In this method the mixture must be dissolved in certain fluid that is called as mobile phase. This mobile phase carries different components of mixture on the structure holding another material. This material is known as stationary phase. There are various types of chromatography methods such as column chromatography, thin layer chromatography, gas chromatography etc.
Answer to Problem 1Q
The large spot concludes that a lot of sample moves up in the plate that makes difficult to analyze the separation.
Explanation of Solution
In TLC technique or Thin layer chromatography, the solvent is used to separate the components of a mixture. The polarity of solvent plays an important role here. If the solvent has high polarity, all components will move along with it and cause large spot with no separation. Similarly if the solvent has low polarity, the components of mixture will not move that causes incomplete separation of them.
Hence large spot concludes that a lot of sample moves up in the plate that makes difficult to analyze the separation.
Thus, the large spot concludes that a lot of sample moves up in the plate that makes difficult to analyze the separation.
Want to see more full solutions like this?
Chapter 10 Solutions
EBK A SMALL SCALE APPROACH TO ORGANIC L
- 4. Enumerate the differences between a serological and a volumetric pipette. 5. Enumerate the uses, advantages, and limitations of a glass versus a plastic volumetric pipette.arrow_forwardCHROMATOGRAPHY HW 1. What does adsorb mean? 2. What is chromatography used for? 3. Why must a pencil rather than a pen be used to mark the TLC plate? 4.If you make an error recording data, how would you correct it? Is it ok to erase it or white it out? 5.Using the data on the last slide, calculate the Rf value for each spot.arrow_forwardWhat is the importance of paper chromotography? What is the usage of paper chromotography in the food industry? Who invented paper chromotography?arrow_forward
- In a warm environment, which restorative wax is recommended for surface restoration? a. opaque b. wouund filler c. lip wax d. medium consistencyarrow_forwardWhose method should be used for the mass production of black ink? What is the retention factor (Rf) of the most polar and least polar spot present in the standard ink?arrow_forwardChemistry What are the major differences between GC and HPLC, discuss the types of samples among other differences.arrow_forward
- Is there any difference between post-processing and post-treatment?arrow_forwardWhat is chiral chromatography?arrow_forwardThe chemical which reacts with HCHO to produce a harmless material called urotropin is ___. This can be used to neutralize a large spill of HCHO. cavity fluid ammonia paraformaldehyde bleacharrow_forward
- The solvent level in the development chamber should be above the level of the spotted compounds on the TLC plate. Group of answer choices True Falsearrow_forwardWhat are the major differences between GC and HPLC, discus the types of samples among other differencesarrow_forwardA) What are the basic principles underlying TLC technique? B) Draw a TLC plate and explain how TLC can be used to identify the presence of a specific compound in an organic mixture.arrow_forward
- EBK A SMALL SCALE APPROACH TO ORGANIC LChemistryISBN:9781305446021Author:LampmanPublisher:CENGAGE LEARNING - CONSIGNMENTMacroscale and Microscale Organic ExperimentsChemistryISBN:9781305577190Author:Kenneth L. Williamson, Katherine M. MastersPublisher:Brooks ColePrinciples of Instrumental AnalysisChemistryISBN:9781305577213Author:Douglas A. Skoog, F. James Holler, Stanley R. CrouchPublisher:Cengage Learning