Essentials of Genetics (9th Edition) - Standalone book
9th Edition
ISBN: 9780134047799
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Concept explainers
Textbook Question
Chapter 1, Problem 10PDQ
Outline the roles played by restriction enzymes and vectors in cloning DNA.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solutionStudents have asked these similar questions
Describe the important features of cloning vectors. Explain the purpose of selectable markers in cloning experiments.
Describe the various types of vectors used in DNA cloning. Explain the difference between a cloning vector and an expression vector.
Explain the process of Cloning DNA into smaller vectors ?
Chapter 1 Solutions
Essentials of Genetics (9th Edition) - Standalone book
Ch. 1 -
1. Describe Mendel’s conclusions about how...Ch. 1 -
2. Review the Chapter Concepts list on p. 1....Ch. 1 - What is the chromosome theory of inheritance, and...Ch. 1 - Prob. 4PDQCh. 1 -
5. Given the state of knowledge at the time of...Ch. 1 - Contrast chromosomes and genes.Ch. 1 - How is genetic information encoded in a DNA...Ch. 1 - Describe the central dogma of molecular genetics...Ch. 1 - How many different proteins, each with a unique...Ch. 1 - Outline the roles played by restriction enzymes...
Ch. 1 - What are some of the impacts of biotechnology on...Ch. 1 - Prob. 12PDQCh. 1 - We all carry about 20,000 genes in our genome. So...Ch. 1 - How has the use of model organisms advanced our...Ch. 1 - If you knew that a devastating late-onset...Ch. 1 -
16. Why do you think discoveries in genetics...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- b) Describe how DNA is digested by different restriction enzymes c) Describe how gel electrophoresis is used to estimate the size of DNA fragments.arrow_forwardExplain the role of restriction endonucleases in the process of genetic engineering.arrow_forwardList all steps involved in a cloning workflow. Name and link the appropriate enzymes to the steps at which they are used.arrow_forward
- With the use of well-illustrated diagrams, reconstruct the entire cloning process by explaining different stages of the cloning process that involves the following:a. Isolation of target DNA fragments (often referred to as inserts)b. Ligation of inserts into the plasmid, creating recombinant molecules c. Transformation of recombinant plasmids into bacteria or other suitable host for propagationd. Screening/selection of hosts containing the intended recombinant plasmid. For this stage(d), discuss the importance of a second marker that can be used for screening of genomic DNA for colonies containing the pka-1 under the principle of insertional inactivation. This should be properly explained using all the attributes of the plasmid described above.arrow_forwardDescribe in vitro mutagenesis and its potential uses.arrow_forwardExplain why a cloning vector usually includes a selectable marker.arrow_forward
- Describe the process of positional cloning and how itallows mapping of disease-causing mutations.arrow_forwardBriefly present experimental and practical benefits of using PCR in DNA cloning process. Give some examples of clinical applications.arrow_forwardDescribe how Restriction Enzymes, Plasmids, DNA Hybridization, and PCR can be used for molecular cloning and genetic engineering.arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
- Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSONBiology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStaxAnatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
- Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & CompanyLaboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education
Molecular Techniques: Basic Concepts; Author: Dr. A's Clinical Lab Videos;https://www.youtube.com/watch?v=7HFHZy8h6z0;License: Standard Youtube License