Why is it best to use sterile distilled water in the preparation of microbial suspension and dry smears for slide preparation? What possible error could happen if a glass slide is reused for slide preparation?
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Why is it best to use sterile distilled water in the preparation of microbial suspension and dry smears for slide preparation? What possible error could happen if a glass slide is reused for slide preparation?
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- What is the physical appearance (solid, liquid, semi-solid) of following media: agar plateWhat is the physical appearance (solid, liquid, semi-solid) of following media:Agar deep tubeFor preparation of a smear on a slide, what is the purpose of heat fixation? What problems can arise when the slide is heated in a flame?
- Why is the looping out method preferable for sputum specimen for acid fast smears?In preparing a bacterial smear for staining, heat fixation is done after the smear dries up.a.) Give the purpose of heat fixation.b.) What can be observed in wet mounts or hanging-drop slides that cannot be observed in heat-fixed slides?Why is heat-fixed procedure in bacterial smear preparation not ideal for capsular staining?
- Why is dilution a necessary part of pure culture preparation?What are some methods to prevent contamination during streak plating a medium?Disinfectants and Sterilization Procedures Complete the table by putting (+) for growth of microorganisms or (–) absence of growth of microorganisms. Disinfectant Concentration Growth after time of exposure (minutes) Control 5 minutes 10 minutes 15 minutes Sodium hypochlorite 5% Sodium hypochlorite 0.05% Alcohol 70% Alcohol 40% Iodophor (Betadine) 10% solution Mouthwash Cetylpyridinium Chloride - 0.075% Sodium Fluoride - 0.05%
- Serial dilution is recommended prior to pour and spread plate. Why is this so?Which of the following is/are true regarding the acid-fast stain? (There may be more than one correct answer.) Non-acid-fast microbes appear blue in a completed acid-fast stain. O Acid-fast cells retain the primary dye after treatment with acid-alcohol. O It is used to identify members of the genus Mycobacterium. V Acid-fast cells appear bright pink/red in a completed acid-fast stain. O If cells are acid-fast, they are gram-negative.Provide three reasons why the use of aseptic technique is essential when handling microbial cultures in the laboratory.