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Why is heat-fixed procedure in bacterial smear preparation not ideal for capsular staining?
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- Why is it essential that smears be air-dried? Why can’t they be gently heated over a flame to speed up the drying process?In preparing a bacterial smear for staining, heat fixation is done after the smear dries up.a.) Give the purpose of heat fixation.b.) What can be observed in wet mounts or hanging-drop slides that cannot be observed in heat-fixed slides?Why must heat or a surface-active agent be used with application of the primary stain during acid-fast staining?
- What are the pros and cons of using the TCE stain-free method versus a traditional gel staining method (CBB staining)?What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and counter stain of this technique? Do we heat fix in here? Describe the organism with purple color. Describe the organism with pinkish red color.Is it possible to perform double or triple staining using fluorescent dyes? Explain.
- Which of the following is/are true regarding the acid-fast stain? (There may be more than one correct answer.) Non-acid-fast microbes appear blue in a completed acid-fast stain. O Acid-fast cells retain the primary dye after treatment with acid-alcohol. O It is used to identify members of the genus Mycobacterium. V Acid-fast cells appear bright pink/red in a completed acid-fast stain. O If cells are acid-fast, they are gram-negative.In the preparation of a bacterial smear, why is there a need to fix the bacteria to the slide? Aside from passing the slide over a flame, what are the other ways of fixing the bacteria to the slide?Why are basic dyes more effective for bacterial staining than acidic dyes? State two ways that can confirm whether a bacterial smear has been correctly prepared or not. Why should you be careful not to underheat a smear during the heat-fixing process? Why do you think the presence of grease or dirt on a glass slide will result in a poor smear preparation? Cite two or three reasons.
- Why is dilution a necessary part of pure culture preparation?List the step-by-step procedure of Gram staining.Jessica placed crystal violet ,grama's iodine and safranin reagents on her bench.Which staining procedure is she preparing for with these reagents? a)Grama's staining procedure b)simple staining procedure c)Acid -fast staining procedure d)Non -acid fast staining procedure e)Scaeffer -Fulton staining procedure