Why is a blank used in the spectrophotometer before the actual pigment is tested?

Q: I have a microscope with oculars that are 20x and am viewing a slide using oculars that are 50x.…

A: Introduction  An instrument used to magnify small objects called a microscope. Even at the cellular…

Q: If 5X instead of 10X oculars were used with the same objectives now on your microscope, what…

A: Microscopy means the use of microscope for studying the cells. Microscope is an indispensable…

Q: A researcher was measuring the absorbance of yellow food coloring. What wavelength should he use on…

A: Light is a form of radiant energy, it is a narrow band of energy within the continuous…

Q: How would you determine the contents of the test tubes if all of the samples were transparent?

A: The study of chemical reactions that occurs within living organisms is called biochemistry. This…

Q: What total magnification will be achieved if the 10× eyepiece and the 100× objective are…

A: The microscope is an instrument which used to view and observe tiny organism or an object that…

Q: When using the oil immersion lens, what four procedures can be implemented to achieve the maximum…

A: Microscopes are useful for viewing objects that are too small to see clearly without magnification.…

Q: Why is there a need to use a staining agent in observing the specimen?

A: Staining agent:          The things that owes-out stains were recognized as staining agent,…

Q: When it comes to simple stains, why is time so important? And what is the purpose of rinsing the…

A: Microbiology is a hugely important field in both basic and applied research. New and fascinating…

Q: Compare AAS, AES and UV-Vis spectrophotometer

A: The most sophisticated spectrophotometers are AAS, AES and UV-vis spectrophotometers. They each have…

Q: What are the most commonly used methods in staining? why?

A: Introduction  Staining is a technique for enhancing contrast in material, usually on a tiny scale.…

Q: What is shown? Why did we use the slide? What is the arrow pointing to? What did we use it for? What…

A: Microscope is a instrument which is used to visualise the specimen that can't be seen by naked eyes…

Q: What are two things that can be done to improve contrast on a microscope?

A: Contrast refers to the darkness of the background relative to the specimen.

Q: What is the total magnification of a specimen viewed with a 10x ocular lens and a 45x objective…

A: The correct ans is 450X. The total magnification of specimen viewed with a 10x ocular lens and 45x…

Q: When using the 40X objective, the total magnification is

A: The total magnification of microscope must be calculated by multiplying the magnification of eye…

Q: If the total magnification is 2000X with the use of a 10X ocular lens, what is the magnification of…

A: Answer- 200X

Q: What is the secondary stain in both acid-fast stain methods?

A: The bacteria is the unicellular prokaryotic organism that maintains a definite shape since it…

Q: what is the total magnification if the objective lens is 4x and the ocular lens is 10x?

A: Step 1 Microscopes are instruments that are used for observing small objects and finer internal…

Q: Why don’t most light microscopes use 303 ocular lenses for greater magnification?

A: The ‘human eye’ is the most valuable and sensitive sense organ. It is a natural optical instrument.…

Q: Discuss the concept of spectrophotometry, colorimetery, and turbidimetry. Maximum of 150 words

A: Spectrophotometry is a part of electromagnetic spectroscopy worried about the quantitative…

Q: Dropping Mercury Electrode

A:

Q: If the ocular lens of a microscope has 10X magnifying power, and the 40X objective lens is being…

A: Microscope  is an instrument used to visualize minute objects . It  consists of two types of lens…

Q: In confocal microscopy, what is the theoretical resolving power of the objective used (63X, NA 1.3,…

A: Resolving power signifies the smallest distance between two separate points of an object, when…

Q: What is the total magnification possible with a 10x objective?

A: The microscope is used to view the objects that are not seen through the naked eye. Microscopes use…

Q: What is the main application of Atomic absorption spectrophotometer (AAS)?

A: The Atomic Absorption Spectrophotometer (AAS) is primarily used to quantify the presence of several…

Q: What do you mean by Auxanometer?

A: The process of plant development can be easily studied by the activity of the meristem. The…

Q: What is the significance of putting scale bar in photomicrographs?

A: Scale bar:A scale bar is an instrument or software that attaches to a slide or image when it is…

Q: Why is it necessary to use contrasting stains while staining

A: Introduction:- Cell staining is a technique that allows you to see cells and cell components more…

Q: What is an electron micrograph? Why do electron micrographshave greater resolution than light…

A: Microscope helps to observe the organisms or objects that cannot be seen by our naked eye because…

Q: spectrophotometer converts light energy into electrical energy?

A: ANS-1 Option B Explanation- Visible light is the only light our eyes can see. Our eyes detect these…

Q: What solution goes into the cuvette when you’re zeroing the spectrophotometer?

A: Spectrophotometer is a device that is used to calculate the absorbance of the light passing through…

Q: If the total magnification of a slide is 400x and the ocular lenses are 10x, what is the magnifying…

A: Magnification is the process of enlarging the apparent size. This enlargement is quantified by a…

Q: what effect would failing to drain the smear in-between staining have on your judgment of the gram…

A: Gram staining is a technique used to differentiate between two groups of bacteria namely…

Q: Do the calibration factor computed varies according to different magnification (objective lenses…

A: By simply accounting for the inaccuracy, the Correction Factor allows us to attain more accurate…

Q: Total magnification of a specimen using the high objective and the ocular lens is

A: B) 40Ox 400 x is the typical magnification of an ocular lens.

Q: what objective lens is the oil objective lens?

A: We have to determine the objective lens that is used for oil immersion.

Q: If the total magnification of a microscope is 700X and the ocular lens magnifies by a factor of 10x,…

A: According to the question, the total magnification of a microscope is 700X.…

Q: What is the principle behind a UV-Vis spectrophotometer, and what are its key components, and how…

A: UV-vis spectrophotometry or UV spectroscopy types of absorption spectroscopy or reflectance…

Q: Why do you use oil on the slide to be examined with the oil-immersion objective?

A: Oil immersion microscopy increases the refractive index of a specimen when used in the correct way.…

Q: What is the differences between ocular magnification and objective magnification?

A: Introduction  A microscope's objective lens is the one near the sample at the bottom. It's an…

Q: Describe the procedure for performing a Gram stain. ▪ What is the procedure? ▪ How does each…

A: Bacteria are ubiquitous microscopic , one celled organism which possess variety of shape ,…

Q: Why sometimes specimen may show organisms under the microscope but not appear in the culture media?

A: Introduction  We are surrounded by various pathogens such as bacteria, viruses, fungus etc. Bacteria…

Q: What is the hazard of the spattering tendency in heating

A: Sterilization is the process of cleaning a substance by removing, killing or deactivating all forms…

Q: How do you know the range in microliters within which a given micropipette can accurately measure?

A: A micropipette is a device used to transfer small volume of samples . It is a vital part in…

Q: Rowena was tasked to measure the actual magnification of her specimen mounted on a slide without…

A: A microscope is a piece of scientific equipment which magnifies very small objects that are too…

Q: Why is it necessary to use the fine adjustment knob with higher magnification?

A: Microscopy is an analytical technique used in all fields of life science. It is used to magnify and…

Q: why is it necessary to use oil immersion on oil objective ?

A: The oil immersion is the technique of light microscopy, which is used to increase the resolving…

Q: What is the stationary and mobile phase of spectrophotometry?

A: Introduction A biotechnique based on the principle of quantitative measurement of light intensity…

Q: Why would specimens viewed with a compound microscope be thin and/or chemically cleared?

A: The human eye can see objects upto 0.1 millimeters. If the objects are smaller than this, the human…

Q: What is the purpose of examining your specimen slide using low power objective first nefore high…

A: Objective lens are magnifying lens that functions to magnify an image. These lens make an important…