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Discuss the concept of spectrophotometry, colorimetery, and turbidimetry.
Maximum of 150 words
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Solved in 4 steps
- the figure shows the result of the spectral analyzer for paper spectrophotometer without any transparent paper and with transparent yellow color paper. observe and discuss the intensity and the wavelength for both results.Explain the relationship between absorbance (A) and transmittance (T) for the basic spectrophotometer technique.What is the area of field of view in micrometers on 400x magnification. Please show work.
- If the ocular lens of a microscope has 10X magnifying power, and the 40X objective lens is being used for observation, what is the total magnification for the viewed image? 10x 43x 1000x 400xIn spectrophotometry, what is the role of the green background in the set-up? What does it represent in the actual spectrophotometer?What is the diameter of the field of view (DFV) of a 1000x objective lens if the DFV of a 400x objective lens is 500 μ? Express your answer in mm.
- Which of the following is not required for a UV-vis absorption spectrophotometer? Group of answer choices camera detector monochromator light sourceWhat is the reason for using blind tube in spectrophotometric and calorimetric studies?If the total magnification of a microscope is 700X and the ocular lens magnifies by a factor of 10x, what is the magnification of the objective lens that was used? Paragraph BIU
- If the field diameter is 2.4 mm and there are 16 cells across the field of view when using the 40X objective, what is the true length of one cell in millimeters (mm)?The field of view (FOV) is the entire circular image we see when looking into the eyepiece. The diameter of the FOV gets smaller as we increase magnification. It can be measured by using a stage micrometer like a ruler, measuring from edge to edge. Notice that the stage micrometer is 1000 microns (µm) in length, and the field of view under the lowest magnification is 5000 µm. Describe how we measure it?How can a UV-Vis spectrophotometer be applicable in determining the absorbance/concentration of a colorless sample? Please answer in detail and clearly. Thank you so much!
